CyFlow™ CD80 Pacific Blue™
| Alternative Name: | B7- B7-1 , BB1 |
| Antibody: | Yes |
| Antigen: | CD80 |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | MEM-233 |
| Emission Maximum: | 455 nm |
| Excitation Maximum: | 400 to 410 nm |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | Pacific Blue™ |
| Isotype: | IgG1 |
| Laser: | Violet |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Human |
| Product number: | BM153215 |
For Research Use Only
| Quantity | 100 tests |
| Volume | 0.4 mL |
| Immunogen | Extracellular domain of human CD80 fused to human IgG1(Fc) |
| Background Information | CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CD152 (CTLA-4). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Vasilevko V, Ghochikyan A, Holterman MJ, Agadjanyan MG: CD80 (B7‑1 and CD86 (B7‑2 are functionally equivalent in the initiation and maintenance of CD4+ T‑cell proliferation after activation with suboptimal doses of PHA. DNA Cell Biol. 2002 Mar; 21(3):137‑49. < PMID: 12015893 > | Zhan H, Towler HM, Calder VL: The immunomodulatory role of human conjunctival epithelial cells. Invest Ophthalmol Vis Sci. 2003 Sep; 44(9):3906‑10. < PMID: 12939308 > | Yadav D, Judkowski V, Flodstrom-Tullberg M, Sterling L, Redmond WL, Sherman L, Sarvetnick N: B7‑2 (CD86 controls the priming of autoreactive CD4 T cell response against pancreatic islets. J Immunol. 2004 Sep 15; 173(6):3631‑9. < PMID: 15356107 > | Tan PH, Beutelspacher SC, Wang YH, McClure MO, Ritter MA, Lombardi G, George AJ: Immunolipoplexes: an efficient, nonviral alternative for transfection of human dendritic cells with potential for clinical vaccination. Mol Ther. 2005 May; 11(5):790‑800. < PMID: 15851017 > | Campioni D, Moretti S, Ferrari L, Punturieri M, Castoldi GL, Lanza F: Immunophenotypic heterogeneity of bone marrow‑derived mesenchymal stromal cells from patients with hematologic disorders: correlation with bone marrow microenvironment. Haematologica. 2006 Mar; 91(3):364‑8. < PMID: 16531259 > | Kolar GR, Mehta D, Pelayo R, Capra JD: A novel human B cell subpopulation representing the initial germinal center population to express AID. Blood. 2007 Mar 15; 109(6):2545‑52. < PMID: 17132718 > | Thomas IJ, Petrich de Marquesini LG, Ravanan R, Smith RM, Guerder S, Flavell RA, Wraith DC, Wen L, Wong FS: CD86 has sustained costimulatory effects on CD8 T cells. J Immunol. 2007 Nov 1; 179(9):5936‑46. < PMID: 17947667 > | Lee DJ, Sieling PA, Ochoa MT, Krutzik SR, Guo B, Hernandez M, Rea TH, Cheng G, Colonna M, Modlin RL: LILRA2 activation inhibits dendritic cell differentiation and antigen presentation to T cells. J Immunol. 2007 Dec 15; 179(12):8128‑36. < PMID: 18056355 > | Eri R, Kodumudi KN, Summerlin DJ, Srinivasan M: Suppression of colon inflammation by CD80 blockade: evaluation of two murine models of inflammatory bowel disease. Inflamm Bowel Dis. 2008 Jan 9; < PMID: 21061388 > | Hovden AO, Karlsen M, Jonsson R, Aarstad HJ, Appel S: Maturation of monocyte derived dendritic cells with OK432 boosts IL‑12p70 secretion and conveys strong T‑cell responses. BMC Immunol. 2011 Jan 5; 12:2. < PMID: 21208424 > | Silk KM, Leishman AJ, Nishimoto KP, Reddy A, Fairchild PJ: Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype. J Biomed Biotechnol. 2012; 2012:172420. < PMID: 22505805 > | Derdak SV, Kueng HJ, Leb VM, Neunkirchner A, Schmetterer KG, Bielek E, Majdic O, Knapp W, Seed B, Pickl WF: Direct stimulation of T lymphocytes by immunosomes: virus‐like particles decorated with T cell receptor/CD3 ligands plus costimulatory molecules. ProcNatlAcadSciUSA. 2006 Aug 29; 103(35):13144‐9. < PMID: 16924110 >