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CyFlow™ CD79a PE

CyFlow™ CD79a PE
Alternative Name: IgA , MB-1, MB1
Antibody: Yes
Antigen: CD79a
Application: Flow cytometry
Clonality: monoclonal
Clone: HM47
Emission Maximum: 576 nm
Excitation Maximum: 496 nm, 565 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: PE
Isotype: IgG1
Laser: Blue , Green, Yellow
Regulatory Status: RUO
Source Species: Mouse
Target Species: Chicken, Cow, Dog, Guinea pig, Horse, Human, Mouse, Non-Human Primates, Pig, Rabbit, Rat
Product number: CU479884

For Research Use Only

$255.00 USD*

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Description Documentation TDS & SDS
Description
Quantity 100 tests Volume 1.0 mL Immunogen Synthetic peptide corresponding to C... more
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CyFlow™ CD79a PE

Quantity100 tests
Volume1.0 mL
ImmunogenSynthetic peptide corresponding to C terminal amino acids 208-222 of human CD79a
Background InformationCD79a (Ig α, MB1) forms disulfide-linked heterodimer with CD79b (Ig β, B29). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with λ5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. At the plasma cell stage, CD79a is present as an intracellular component. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Mason DY, Cordell JL, Tse AG, van Dongen JJ, van Noesel CJ, Micklem K, Pulford KA, Valensi F, Comans-Bitter WM, Borst J, et al: The IgM‑associated protein mb‑1 as a marker of normal and neoplastic B cells. J Immunol. 1991 Dec 1; 147(11):2474‑82. < PMID: 1747162 > | Rassenti LZ, Kipps TJ: Expression of Ig‑beta (CD79b) by chronic lymphocytic leukemia B cells that lack immunoglobulin heavy‑chain allelic exclusion. Blood. 2000 Apr 15; 95(8):2725‑7. < PMID: 10753858 > | Torlakovic E, Torlakovic G: B‑cell markers in lymphocyte predominance Hodgkin disease. Arch Pathol Lab Med. 2002 Jul; 126(7):862‑3. < PMID: 12123231 > | Islas-Ohlmayer M, Padgett-Thomas A, Domiati-Saad R, Melkus MW, Cravens PD, Martin Mdel P, Netto G, Garcia JV: Experimental infection of NOD/SCID mice reconstituted with human CD34+ cells with Epstein‑Barr virus. J Virol. 2004 Dec; 78(24):13891‑900. < PMID: 15564497 > | Fernandez NJ, West KH, Jackson ML, Kidney BA: Immunohistochemical and histochemical stains for differentiating canine cutaneous round cell tumors.. Vet Pathol. 2005 Jul; 42(4):437‑45. < PMID: 16006603 > | Bhargava P, Kallakury BV, Ross JS, Azumi N, Bagg A: CD79a is heterogeneously expressed in neoplastic and normal myeloid precursors and megakaryocytes in an antibody clone‑dependent manner. Am J Clin Pathol. 2007 Aug; 128(2):306‑13. < PMID: 17638667 > | Zhao XF, Hassan A, Perry A, Ning Y, Stass SA, Dehner LP: C‑MYC rearrangements are frequent in aggressive mature B‑Cell lymphoma with atypical morphology. Int J Clin Exp Pathol. 2008 Jan 1; 1(1):65‑74. < PMID: 18784824 >

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