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CyFlow™ CD72 PE

CyFlow™ CD72 PE
Alternative Name: Lyb-2 , LYB2
Antibody: Yes
Antigen: CD72
Application: Flow cytometry
Clonality: monoclonal
Clone: 3F3
Emission Maximum: 576 nm
Excitation Maximum: 496 nm, 565 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: PE
Isotype: IgG2b
Laser: Blue , Green, Yellow
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: AA664263

For Research Use Only

$255.00 USD*

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Description
Quantity 100 tests Volume 2.0 mL Immunogen Normal human lymphocytes from lymph node... more
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CyFlow™ CD72 PE
Quantity100 tests
Volume2.0 mL
ImmunogenNormal human lymphocytes from lymph node
Background InformationCD72 is a transmembrane glycoprotein expressed as a homodimer especially in B cells, but also in other antigen presenting cells such as dendritic cells and macrophages. Through one of its immunoreceptor tyrosine-based inhibitory motives (ITIMs), CD72 interacts with tyrosine phosphatase SHP-1, thereby suppressing B cell responsiveness. Binding of CD72 with its ligand CD100 (Sema4D) prevents BCR association and phosphorylation of CD72 and results in dissociation of SHP-1 from CD72, thus enables B cell activation.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J Clin Pathol. 1993 Apr; 46(4):334‑6. < PMID: 7684403 > | Kumanogoh A, Watanabe C, Lee I, Wang X, Shi W, Araki H, Hirata H, Iwahori K, Uchida J, Yasui T, Matsumoto M, Yoshida K, Yakura H, Pan C, Parnes JR, Kikutani H: Identification of CD72 as a lymphocyte receptor for the class IV semaphorin CD100: a novel mechanism for regulating B cell signaling. Immunity. 2000 Nov; 13(5):621‑31. < PMID: 11114375 > | Kumanogoh A, Kikutani H: The CD100‑CD72 interaction: a novel mechanism of immune regulation. Trends Immunol. 2001 Dec; 22(12):670‑6. < PMID: 11738997 > | Kish DD, Gorbachev AV, Fairchild RL: CD8+ T cells produce IL‑2, which is required for CD(4+)CD25+ T cell regulation of effector CD8+ T cell development for contact hypersensitivity responses. J Leukoc Biol. 2005 Sep; 78(3):725‑35. < PMID: 16000396 > | Kumanogoh A, Shikina T, Watanabe C, Takegahara N, Suzuki K, Yamamoto M, Takamatsu H, Prasad DV, Mizui M, Toyofuku T, Tamura M, Watanabe D, Parnes JR, Kikutani H: Requirement for CD100‑CD72 interactions in fine‑tuning of B‑cell antigen receptor signaling and homeostatic maintenance of the B‑cell compartment. Int Immunol. 2005 Oct; 17(10):1277‑82. < PMID: 16113236 > | Mizrahi S, Markel G, Porgador A, Bushkin Y, Mandelboim O: CD100 on NK cells enhance IFNgamma secretion and killing of target cells expressing CD72. PLoS One. 2007 Sep 5; 2(9):e818. < PMID: 17786190 > | Mizrahi S, Markel G, Porgador A, Bushkin Y, Mandelboim O: CD100 on NK cells enhance IFNgamma secretion and killing of target cells expressing CD72. PLoS One. 2007 Sep 5; 2(9):e818. < PMID: 17786190 > | Dauby N, Kummert C, Lecomte S, Liesnard C, Delforge ML, Donner C, Marchant A: Primary Human Cytomegalovirus Infection Induces the Expansion of Virus‑Specific Activated and Atypical Memory B Cells. J Infect Dis. 2014 May 1; < PMID: 25958387 >

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