CyFlow™ CD64 Low Endotoxin
| Alternative Name: | FcγRI |
| Antibody: | Yes |
| Antigen: | CD64 |
| Application: | Flow cytometry, Functional assays, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western blot |
| Clonality: | monoclonal |
| Clone: | 10.1 |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | Low Endotoxin |
| Isotype: | IgG1 |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Human, Non-Human Primates |
| Product number: | AR810750 |
For Research Use Only
| HLDA Workshop | HLDA III—WS Code M-250 |
| Concentration Unit | mg/mL |
| Concentration | 1 |
| Quantity | 0.1 mg |
| Volume | 0.1 mL |
| Immunogen | Rheumatoid synovial fluid cells and fibronectin purified human monocytes |
| Background Information | CD64 (FcγRI) is a cell surface receptor for Fc region of IgG. It is composed of specific ligand binding α subunit and promiscuous γ subunit, which is indispensable for tyrosine-based signaling. However, even the α subunit can transduce signals leading to cellular effector functions. The isoform FcγRIa1 binds human IgG with high affinity, has limited myeloid cell distribution, and a relatively large intracellular domain. Products of related genes include FcγRIb and FcγRIc isoforms, but these specify low affinity IgG receptors if functionally expressed at all. Besides a role in antigen clearance, FcgammaRI (a1) can potently enhance MHC class I and II antigen presentation in vitro and in vivo. |
| Storage Buffer | The reagent is provided in azide-free phosphate buffered saline (PBS) solution, pH ≈7.4; 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
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