CyFlow™ CD62L Alexa Fluor™ 700
| Alternative Name: | L-selectin |
| Antibody: | Yes |
| Antigen: | CD62L |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | DREG56 |
| Emission Maximum: | 723 nm |
| Excitation Maximum: | 702 nm |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | Alexa Fluor™ 700 |
| Isotype: | IgG1 |
| Laser: | Red |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Human |
| Product number: | AU807163 |
For Research Use Only
| Quantity | 100 tests |
| Volume | 0.4 mL |
| Immunogen | PMA-activated human peripheral blood leukocytes |
| Background Information | CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1). The level of CD62L expression can be used to help distinguish naive T cells from effector/memory T cells. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
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