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CyFlow™ CD59 Azide Free

CyFlow™ CD59 Azide Free
Alternative Name: Protectin H19
Antibody: Yes
Antigen: CD59
Application: Flow cytometry, Functional assays
Clonality: monoclonal
Clone: MEM-129
Field of Interest: Immunophenotyping
Format/Fluorochrome: Azide Free
Isotype: IgM
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human, Pig
Product number: BR267721

For Research Use Only

$120.00 USD*

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Description Documentation TDS & SDS
Description
Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL... more
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CyFlow™ CD59 Azide Free
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenHuman peripheral blood lymphocytes
Background InformationCD59 (Protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). It is thus the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 associates with C5b-8 complex and thereby counteracts appropriate formation of cytolytic pore within the plasma membrane. CD59 is also an low-affinity ligand of human CD2 and causes T cell costimulation.
Storage BufferThe reagent is provided in azide-free HEPES buffered saline (HBS) solution, pH ≈7.0; 0.2 µm filter sterilized.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Meri S, Morgan BP, Davies A, Daniels RH, Olavesen MG, Waldmann H, Lachmann PJ: Human protectin (CD59), an 18,000‑20,000 MW complement lysis restricting factor, inhibits C5b‑8 catalysed insertion of C9 into lipid bilayers. Immunology. 1990 Sep; 71(1):42614. < PMID: 2172297 > | Rooney IA, Davies A, Griffiths D, Williams JD, Davies M, Meri S, Lachmann PJ, Morgan BP: The complement‑inhibiting protein, protectin (CD59 antigen), is present and functionally active on glomerular epithelial cells. Clin Exp Immunol. 1991 Feb; 83(2):251‑6. < PMID: 1704296 > | Menu E, Tsai BC, Bothwell AL, Sims PJ, Bierer BE: CD59 costimulation of T cell activation: CD58 dependence and requirement for glycosylation. J Immunol. 1994 Sep 15; 153(6):2444‑56. < PMID: 7521361 > | Olweus J, Lund-Johansen F, Terstappen LW: CD64/Fc gamma RI is a granulo‑monocytic lineage marker on CD34+ hematopoietic progenitor cells. Blood. 1995 May 1; 85(9):2402‑13. < PMID: 7537112 > | Stulnig TM, Berger M, Sigmund T, Stockinger H, Horejsí V, Waldhäusl W: Signal transduction via glycosyl phosphatidylinositol‑anchored proteins in T cells is inhibited by lowering cellular cholesterol. J Biol Chem. 1997 Aug 1; 272(31):19242‑7. < PMID: 9235917 > | Baalasubramanian S, Harris CL, Donev RM, Mizuno M, Omidvar N, Song WC, Morgan BP: CD59a is the primary regulator of membrane attack complex assembly in the mouse. J Immunol. 2004 Sep 15; 173(6):3684‑92. < PMID: 15356114 >

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