CyFlow™ CD54 PE
| Alternative Name: | ICAM-1 |
| Antibody: | Yes |
| Antigen: | CD54 |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | MEM-111 |
| Emission Maximum: | 576 nm |
| Excitation Maximum: | 496 nm, 565 nm |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | PE |
| Isotype: | IgG2a |
| Laser: | Blue , Green, Yellow |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Cow, Human, Rat |
| Product number: | AD327626 |
For Research Use Only
| HLDA Workshop | HLDA VI—WS Code AS A049 |
| Quantity | 100 tests |
| Volume | 2.0 mL |
| Immunogen | Raji cells: human Burkitt's lymphoma cell line |
| Background Information | CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
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