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CyFlow™ CD54 Biotin

CyFlow™ CD54 Biotin
Alternative Name: ICAM-1
Antibody: Yes
Antigen: CD54
Application: Flow cytometry
Clonality: monoclonal
Clone: 1H4
Field of Interest: Immunophenotyping
Format/Fluorochrome: Biotin
Isotype: IgG2b
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: CH673649

For Research Use Only

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Description
Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL... more
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CyFlow™ CD54 Biotin
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenRaji cells and spleen cells fused with NS1 cells
Background InformationCD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.
UsageThe reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working usage is 5·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Boyd AW, Wawryk SO, Burns GF, Fecondo JV: Intercellular adhesion molecule 1 (ICAM‑1) has a central role in cell‑cell contact‑mediated immune mechanisms. Proc Natl Acad Sci USA. 1988 May; 85(9):3095‑9. < PMID: 3362863 > | Boyd AW, Dunn SM, Fecondo JV, Culvenor JG, Duhrsen U, Burns GF, Wawryk SO: Regulation of expression of a human intercellular adhesion molecule (ICAM‑1) during lymphohematopoietic differentiation. Blood. 1989 May 15; 73(7):1896‑903. < PMID: 2469503 > | Springer TA: Adhesion receptors of the immune system. Nature. 1990 Aug 2; 346(6283):425‑34. < PMID: 1974032 > | Ockenhouse CF, Betageri R, Springer TA, Staunton DE: Plasmodium falciparum‑infected erythrocytes bind ICAM‑1 at a site distinct from LFA‑1, Mac‑1, and human rhinovirus. Cell. 1992 Jan 10; 68(1):63‑9. < PMID: 1346257 > | Williams DT, Chaudhry Y, Goodfellow IG, Lea S, Evans DJ: Interactions of decay‑accelerating factor (DAF) with haemagglutinating human enteroviruses: utilizing variation in primate DAF to map virus binding sites. J Gen Virol. 2004 Mar; 85(3):731‑8. < PMID: 14993659 >

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