CyFlow™ CD48 Biotin
| Alternative Name: | BCM1, BLAST-1 , SLAMF2 |
| Antibody: | Yes |
| Antigen: | CD48 |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | MEM-102 |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | Biotin |
| Isotype: | IgG1 |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Human, Non-Human Primates |
| Product number: | AB716811 |
For Research Use Only
| Concentration Unit | mg/mL |
| Concentration | 1 |
| Quantity | 0.1 mg |
| Volume | 0.1 mL |
| Immunogen | Raji cells: human Burkitt's lymphoma cell line |
| Background Information | CD48 (Blast-1) belongs to the CD2 subset of the Ig superfamily, which includes CD2, CD2F-10, CD58, CD84, CD150, CD229, CD244 and others. These molecules bind to the same or another members of their family, thus mediate homotypic or heterotypic adhesion. CD48 is a GPI-anchored protein broadly expressed on hematopoietic cells and serves as a high affinity ligand for 2B4 and low affinity ligand for CD2. 2B4-CD48 interaction among NK cells and NK-T cells regulates cell proliferation. Signaling through CD48 results in eosinophil activation and CD48 expression is increased in several infectious diseases. |
| Usage | The reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working usage is 1·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator. |
| Storage Buffer | The reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Bazil V, Stefanova I, Hilgert I, Kristofova H, Vanek S, Bukovsky A, Horejsi V: Monoclonal antibodies against human leucocyte antigens (III): Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM‑53 and MEM‑102. Folia Biol (Praha). 1989; 35(5):289‑97. < PMID: 2534480 > | Knapp W, Dorken B, Gilks W, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (Eds): Leucocyte Typing IV. Oxford University Press, Oxford. 1989; 1‑1820. < NLM ID: 8914679 > | Korinek V, Stefanova I, Angelisova P, Hilgert I, Horejsi V: The human leucocyte antigen CD48 (MEM‑102) is closely related to the activation marker Blast‑1. Immunogenetics. 1991; 33(2):108‑12. < PMID: 1999350 > | Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J Clin Pathol. 1993 Apr; 46(4):334‑6. < PMID: 7684403 > | Stulnig TM, Berger M, Sigmund T, Stockinger H, Horejsí V, Waldhäusl W: Signal transduction via glycosyl phosphatidylinositol‑anchored proteins in T cells is inhibited by lowering cellular cholesterol. J Biol Chem. 1997 Aug 1; 272(31):19242‑7. < PMID: 9235917 > | Angelisová P, Drbal K, Horejsí V, Cerný J: Association of CD10/neutral endopeptidase 24.11 with membrane microdomains rich in glycosylphosphatidylinositol‑anchored proteins and Lyn kinase. Blood. 1999 Feb 15; 93(4):1437‑9. < PMID: 10075459 > | Mathew SO, Kumaresan PR, Lee JK, Huynh VT, Mathew PA: Mutational analysis of the human 2B4 (CD244)/CD48 interaction: Lys68 and Glu70 in the V domain of 2B4 are critical for CD48 binding and functional activation of NK cells. J Immunol. 2005 Jul 15; 175(2):1005‑13. < PMID: 16002700 > | Assarsson E, Kambayashi T, Persson CM, Chambers BJ, Ljunggren HG: 2B4/CD48‑mediated regulation of lymphocyte activation and function. J Immunol. 2005 Aug 15; 175(4):2045‑9. < PMID: 16081768 > | Lee KM, Forman JP, McNerney ME, Stepp S, Kuppireddi S, Guzior D, Latchman YE, Sayegh MH, Yagita H, Park CK, Oh SB, Wülfing C, Schatzle J, Mathew PA, Sharpe AH,Kumar V: Requirement of homotypic NK‑cell interactions through 2B4(CD244)/CD48 in the generation of NK effector functions. Blood. 2006 Apr 15; 107(8):3181‑8. < PMID: 15905190 > | Munitz A, Bachelet I, Eliashar R, Khodoun M, Finkelman FD, Rothenberg ME, Levi-Schaffer F: CD48 is an allergen and IL‑3‑induced activation molecule on eosinophils. J Immunol. 2006 Jul 1; 177(1):77‑83. < PMID: 16785501 > | Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single‑molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May; 19(5):675‑84. < PMID: 17446208 >