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CyFlow™ CD45RA Pacific Orange™

CyFlow™ CD45RA Pacific Orange™
Alternative Name: Leu18
Antibody: Yes
Antigen: CD45RA
Application: Flow cytometry
Clonality: monoclonal
Clone: MEM-56
Emission Maximum: 551 nm
Excitation Maximum: 400 nm
Field of Interest: Immunophenotyping, Phosphorylation
Format/Fluorochrome: Pacific Orange™
Isotype: IgG2b
Laser: Violet
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: CF345450

For Research Use Only

$300.00 USD*

Price excludes any applicable taxes plus shipping costs

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Description
HLDA Workshop HLDA IV—WS Code NL 907 Quantity 100 tests Volume 0.4 mL Immunogen... more
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CyFlow™ CD45RA Pacific Orange™

HLDA WorkshopHLDA IV—WS Code NL 907
Quantity100 tests
Volume0.4 mL
ImmunogenHuman thymocytes and T lymphocytes
Background InformationCD45RA (Leu18) is a high molecular weight isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RA is expressed e.g. on naive T cells and normal plasma cells.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Bazil V, Horejsi V, Baudys M, Kristofova H, Strominger JL, Kostka W, Hilgert I: Biochemical characterization of a soluble form of the 53‑kDa monocyte surface antigen. Eur J Immunol. 1986 Dec; 16(12):1583‑9. < PMID: 3493149 > | Knapp W, Dorken B, Gilks W, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (Eds): Leucocyte Typing IV. Oxford University Press, Oxford. 1989; 1‑1820. < NLM ID: 8914679 > | Bhunia AK, Arai T, Bulkley G, Chatterjee S: Lactosylceramide mediates tumor necrosis factor‑alpha‑induced intercellular adhesion molecule‑1 (ICAM‑1 expression and the adhesion of neutrophil in human umbilical vein endothelial cells. J Biol Chem. 1998 Dec 18; 273(51):34349‑57. < PMID: 9852101 > | Dawes R, Petrova S, Liu Z, Wraith D, Beverley PC, Tchilian EZ: Combinations of CD45 isoforms are crucial for immune function and disease. J Immunol. 2006 Mar 15; 176(6):3417‑25. < PMID: 16517710 > | Cortez-Gonzalez X, Sidney J, Adotevi O, Sette A, Millard F, Lemonnier F, Langlade-Demoyen P, Zanetti M: Immunogenic HLA‑B7‑restricted peptides of hTRT. Int Immunol. 2006 Dec; 18(12):1707‑18. < PMID: 17077179 > | Li C, Wong P, Pan T, Xiao F, Yin S, Chang B, Kang SC, Ironside J, Sy MS: Normal cellular prion protein is a ligand of selectins: binding requires Le(X) but is inhibited by sLe(X). Biochem J. 2007 Sep 1; 406(2):333‑41. < PMID: 17497959 > | Desharnais P, Dupéré-Minier G, Hamelin C, Devine P, Bernier J: Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents. Apoptosis. 2008 Feb; 13(2):197‑212. < PMID: 18157742 >

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