Article successfully added.

CyFlow™ CD45R0 APC

CyFlow™ CD45R0 APC
Alternative Name: UCHL-1
Antibody: Yes
Antigen: CD45R0
Application: Flow cytometry
Clonality: monoclonal
Clone: UCHL1
Emission Maximum: 660 nm
Excitation Maximum: 650 nm
Field of Interest: Immunophenotyping, Phosphorylation
Format/Fluorochrome: APC
Isotype: IgG2a
Laser: Red
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: BR164354

For Research Use Only

$255.00 USD*

Price excludes any applicable taxes plus shipping costs

HLDA Workshop HLDA III—WS Code NL 826 HLDA III—WS Code T 128 HLDA IV—WS Code NL 31 HLDA V—WS... more
CyFlow™ CD45R0 APC

HLDA WorkshopHLDA III—WS Code NL 826 HLDA III—WS Code T 128 HLDA IV—WS Code NL 31 HLDA V—WS Code BP BP460 HLDA V—WS Code T T-081 HLDA V—WS Code T T-CD45.43
Quantity100 tests
Volume1.0 mL
ImmunogenHuman IL-2 dependent T cells
Background InformationCD45R0 (UCHL-1) is the shortest isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45R0 is expressed e.g. on macrophages, CD8+ T cells, activated T cells and myeloma cells.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Norton AJ, Ramsay AD, Smith SH, Beverley PC, Isaacson PG: Monoclonal antibody (UCHL{01} that recognises normal and neoplastic T cells in routinely fixed tissues. J Clin Pathol. 1986 Apr; 39(4):399‐405. < PMID: 2422219 > | Smith SH, Brown MH, Rowe D, Callard RE, Beverley PC: Functional subsets of human helper‑inducer cells defined by a new monoclonal antibody, UCHL1. Immunology. 1986 May; 58(1):63‑70. < PMID: 2423439 > | McMichael AJ, Beverley PCL, Cobbold S, et al (Eds): Leucocyte Typing III, White Cell Differentiation Antigens. Oxford University Press, Oxford. 1987; 1‑1050. < NLM ID: 8913266 > | Beverley PC: Human T cell subsets. Immunol Lett. 1987 Apr; 14(4):263‑7. < PMID: 2953677 > | Beverley PC, Merkenschlager M, Terry L: Phenotypic diversity of the CD45 antigen and its relationship to function. Immunol Suppl. 1988; 1:3‑5. < PMID: 2968306 > | Akbar AN, Terry L, Timms A, Beverley PC, Janossy G: Loss of CD45R and gain of UCHL1 reactivity is a feature of primed T cells. J Immunol. 1988 Apr 1; 140(7):2171‑8. < PMID: 2965180 > | Terry LA, Brown MH, Beverley PC: The monoclonal antibody, UCHL1, recognizes a 180,000 MW component of the human leucocyte‑common antigen, CD45. Immunology. 1988 Jun; 64(2):331‑6. < PMID: 2455685 > | Merkenschlager M, Terry L, Edwards R, Beverley PC: Limiting dilution analysis of proliferative responses in human lymphocyte populations defined by the monoclonal antibody UCHL1: implications for differential CD45 expression in T cell memory formation. Eur J Immunol. 1988 Nov; 18(11):1653‑61. < PMID: 2974420 > | Knapp W, Dorken B, Gilks W, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (Eds): Leucocyte Typing IV. Oxford University Press, Oxford. 1989; 1‑1820. < NLM ID: 8914679 > | Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J Clin Pathol. 1993 Apr; 46(4):334‑6. < PMID: 7684403 > | Li FJ, Tsuyama N, Ishikawa H, Obata M, Abroun S, Liu S, Otsuyama K, Zheng X, Ma Z, Maki Y, Kawano MM: A rapid translocation of CD45RO but not CD45RA to lipid rafts in IL‑6‑induced proliferation in myeloma. Blood. 2005 Apr 15; 105(8):3295‑302. < PMID: 15626731 > | Dawes R, Petrova S, Liu Z, Wraith D, Beverley PC, Tchilian EZ: Combinations of CD45 isoforms are crucial for immune function and disease. J Immunol. 2006 Mar 15; 176(6):3417‑25. < PMID: 16517710 > | Cosenza-Nashat MA, Kim MO, Zhao ML, Suh HS, Lee SC: CD45 isoform expression in microglia and inflammatory cells in HIV‑1 encephalitis.. Brain Pathol. 2006 Oct; 16(4):256‑65. < PMID: 17107594 > | Bijian K, Zhang L, Shen SH: Collagen‑mediated survival signaling is modulated by CD45 in Jurkat T cells. Mol Immunol. 2007 Jul; 44(15):3682‑90. < PMID: 17524482 > | Desharnais P, Dupéré-Minier G, Hamelin C, Devine P, Bernier J: Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents. Apoptosis. 2008 Feb; 13(2):197‑212. < PMID: 18157742 >