CyFlow™ CD45R0 APC
| Alternative Name: | UCHL-1 |
| Antibody: | Yes |
| Antigen: | CD45R0 |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | UCHL1 |
| Emission Maximum: | 660 nm |
| Excitation Maximum: | 650 nm |
| Field of Interest: | Immunophenotyping, Phosphorylation |
| Format/Fluorochrome: | APC |
| Isotype: | IgG2a |
| Laser: | Red |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Human |
| Product number: | BR164354 |
For Research Use Only
| HLDA Workshop | HLDA III—WS Code NL 826 HLDA III—WS Code T 128 HLDA IV—WS Code NL 31 HLDA V—WS Code BP BP460 HLDA V—WS Code T T-081 HLDA V—WS Code T T-CD45.43 |
| Quantity | 100 tests |
| Volume | 1.0 mL |
| Immunogen | Human IL-2 dependent T cells |
| Background Information | CD45R0 (UCHL-1) is the shortest isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45R0 is expressed e.g. on macrophages, CD8+ T cells, activated T cells and myeloma cells. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
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