CyFlow™ CD44 Purified
| Alternative Name: | H-CAM , Pgp-1 |
| Antibody: | Yes |
| Antigen: | CD44 |
| Application: | Enzyme-linked immunosorbent assay, Flow cytometry, Immunoprecipitation, Western blot |
| Clonality: | monoclonal |
| Clone: | MEM-85 |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | Purified |
| Isotype: | IgG2b |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Human |
| Product number: | BX199697 |
For Research Use Only
| HLDA Workshop | HLDA IV—WS Code NL 706 HLDA VI—WS Code AS Ref.15 |
| Concentration Unit | mg/mL |
| Concentration | 1 |
| Quantity | 0.1 mg |
| Volume | 0.1 mL |
| Immunogen | Leukocytes of a patient suffering from LGL-type leukemia |
| Background Information | CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumor growth and metastasis, antiapoptosis and directional motility of cancer cells. |
| Storage Buffer | The reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Stefanová I, Hilgert I, Bazil V, Kristofová H, Horejsí V: Human leucocyte surface glycoprotein CDw44 and lymphocyte homing receptor are identical molecules. Immunogenetics. 1989; 29(6):402‑4. < PMID: 2659502 > | Knapp W, Dorken B, Gilks W, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (Eds): Leucocyte Typing IV. Oxford University Press, Oxford. 1989; 1‑1820. < NLM ID: 8914679 > | Bazil V, Strominger JL: Metalloprotease and serine protease are involved in cleavage of CD43, CD44, and CD16 from stimulated human granulocytes: Induction of cleavage of L‑selectin via CD16. J Immunol. 1994 Feb 1; 152(3):1314‑22. < PMID: 7507963 > | Litvinov SV, Velders MP, Bakker HA, Fleuren GJ, Warnaar SO: Ep‑CAM: a human epithelial antigen is a homophilic cell‑cell adhesion molecule. J Cell Biol. 1994 Apr; 125(2):437‑46. < PMID: 8163559 > | Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland Publishing Inc, New York. 1997; 1‑1342. < NLM ID: 9712219 > | Schmidt CS, Liu J, Zhang T, Song HY, Sandusky G, Mintze K, Benschop RJ, Glasebrook A, Yang DD, Na S: Enhanced B cell expansion, survival, and humoral responses by targeting death receptor 6. J Exp Med. 2003 Jan 6; 197(1):51‑62. < PMID: 12515813 > | Suárez L, Vidriales MB, García-Laraña J, Sanz G, Moreno MJ, López A, Barrena S, Martínez R, Tormo M, Palomera L, Lavilla E, López-Berges MC, de Santiago M, de Equiza ME, Miguel JF, Orfao A: CD34+ cells from acute myeloid leukemia, myelodysplastic syndromes, andnormal bone marrow display different apoptosis and drug resistance‑associated phenotypes. Clin Cancer Res. 2004 Nov 15; 10(22):7599‑606. < PMID: 15569991 > | Holleman A, den Boer ML, Cheok MH, Kazemier KM, Pei D, Downing JR, Janka-Schaub GE, Göbel U, Graubner UB, Pui CH, Evans WE, Pieters R: Expression of the outcome predictor in acute leukemia 1 (OPAL1 gene is not an independent prognostic factor in patients treated according to COALL or St Jude protocols. Blood. 2006 Sep 15; 108(6):1984‑90. < PMID: 16709928 > | Kolar GR, Mehta D, Pelayo R, Capra JD: A novel human B cell subpopulation representing the initial germinal center population to express AID. Blood. 2007 Mar 15; 109(6):2545‑52. < PMID: 17132718 > | Ide K, Wang H, Tahara H, Liu J, Wang X, Asahara T, Sykes M, Yang YG, Ohdan H: Role for CD47‑SIRPalpha signaling in xenograft rejection by macrophages. Proc Natl Acad Sci USA. 2007 Mar 20; 104(12):5062‑6. < PMID: 17360380 > | Subramaniam V, Gardner H, Jothy S: Soluble CD44 secretion contributes to the acquisition of aggressive tumor phenotype in human colon cancer cells. Exp Mol Pathol. 2007 Dec; 83(3):341‑6. < PMID: 17945213 > | Viel EC, Lemarié CA, Benkirane K, Paradis P, Schiffrin EL: Immune regulation and vascular inflammation in genetic hypertension. Am J Physiol Heart Circ Physiol. 2010 Mar; 298(3):H938‑44. < PMID: 20044442 > | Kanderova V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, Hrusak O, Lund‐Johansen F, Kalina T: High‐resolution antibody array analysis of childhood acute leukemia cells. Mol Cell Proteomics. 2016 Apr 1; 15(4):1246‐61. < PMID: 26785729 >