CyFlow™ CD44 Biotin
| Alternative Name: | H-CAM , Pgp-1 |
| Antibody: | Yes |
| Antigen: | CD44 |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | IM7 |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | Biotin |
| Isotype: | IgG2b |
| Regulatory Status: | RUO |
| Source Species: | Rat |
| Target Species: | Cat, Dog, Horse, Human, Mouse |
| Product number: | AR623134 |
For Research Use Only
| Concentration Unit | mg/mL |
| Concentration | 1 |
| Quantity | 0.1 mg |
| Volume | 0.1 mL |
| Immunogen | Dexamethasone-induced cells of the SJL mouse spontaneous myeloid leukemia M1 |
| Background Information | CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumor growth and metastasis, antiapoptosis and directional motility of cancer cells. |
| Usage | The reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Working concentrations should be determined by the investigator. |
| Storage Buffer | The reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Lesley J, Trowbridge IS: Genetic characterization of a polymorphic murine cell‑surface glycoprotein. Immunogenetics. 1982 Mar; 15(3):313‑20. < PMID: 7068175 > | Si-Tahar M, Sitaraman S, Shibahara T, Madara JL: Negative regulation of epithelium‑neutrophil interactions via activation of CD44. Am J Physiol Cell Physiol. 2001 Mar; 280(3):C423‑32. < PMID: 11171560 > | Legg JW, Lewis CA, Parsons M, Ng T, Isacke CM: A novel PKC‑regulated mechanism controls CD44 ezrin association and directional cell motility. Nat Cell Biol. 2002 Jun; 4(6):399‑407. < PMID: 12032545 > | McKallip RJ, Do Y, Fisher MT, Robertson JL, Nagarkatti PS, Nagarkatti M: Role of CD44 in activation‑induced cell death: CD44‑deficient mice exhibit enhanced T cell response to conventional and superantigens. Int Immunol. 2002 Sep; 14(9):1015‑26. < PMID: 12202399 > | Xu H, Manivannan A, Liversidge J, Sharp PF, Forrester JV, Crane IJ: Involvement of CD44 in leukocyte trafficking at the blood‑retinal barrier. J Leukoc Biol. 2002 Dec; 72(6):1133‑41. < PMID: 12488494 > | Katoh S, Matsumoto N, Kawakita K, Tominaga A, Kincade PW, Matsukura S: A role for CD44 in an antigen‑induced murine model of pulmonary eosinophilia. J Clin Invest. 2003 May; 111(10):1563‑70. < PMID: 12750406 > | van Royen N, Voskuil M, Hoefer I, Jost M, de Graaf S, Hedwig F, Andert JP, Wormhoudt TA, Hua J, Hartmann S, Bode C, Buschmann I, Schaper W, van der Neut R, Piek JJ, Pals ST: CD44 regulates arteriogenesis in mice and is differentially expressed in patients with poor and good collateralization. Circulation. 2004 Apr 6; 109(13):1647‑5. < PMID: 15023889 > | Larkin J, Renukaradhya GJ, Sriram V, Du W, Gervay-Hague J, Brutkiewicz RR: CD44 differentially activates mouse NK T cells and conventional T cells. J Immunol. 2006 Jul 1; 177(1):268‑79. < PMID: 16785522 > | Hegde VL, Singh NP, Nagarkatti PS, Nagarkatti M: CD44 mobilization in allogeneic dendritic cell‑T cell immunological synapse plays a key role in T cell activation. J Leukoc Biol. 2008 Jul; 84(1):134‑42. < PMID: 18388297 >