CyFlow™ CD43 FITC
| Alternative Name: | Leukosialin , sialophorin |
| Antibody: | Yes |
| Antigen: | CD43 |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | MEM-59 |
| Emission Maximum: | 518 nm |
| Excitation Maximum: | 490 to 495 nm |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | FITC |
| Isotype: | IgG1 |
| Laser: | Blue |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Human |
| Product number: | BR081923 |
For Research Use Only
| HLDA Workshop | HLDA IV—WS Code NL 604 HLDA V—WS Code AS S290 |
| Quantity | 100 tests |
| Volume | 2.0 mL |
| Immunogen | Human T lymphocytes |
| Background Information | CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation. By interaction with actin-binding proteins ezrin and moesin, CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic. CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 causes induction of functionally active tumor suppressor p53 protein, but in case of p53 and ARF defficiency CD43 promotes tumour proliferation and viability. It appears to be an important modulator of leukocyte functions. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Stefanova I, Hilgert I, Angelisova P, Kristofova H, Horejsi V: Characterization of a 95 kDa human leucocyte sialoglycoprotein: its identity with CD43, gpL115, leukosialin and sialophorin. Folia Biol (Praha). 1988; 34(4):255‑65. < PMID: 2467832 > | Knapp W, Dorken B, Gilks W, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (Eds): Leucocyte Typing IV. Oxford University Press, Oxford. 1989; 1‑1820. < NLM ID: 8914679 > | Stefanova I, Hilgert I, Kristofova H, Brown R, Low MG, Horejsi V: Characterization of a broadly expressed human leucocyte surface antigen MEM‑43 anchored in membrane through phosphatidylinositol. Mol Immunol. 1989 Feb; 26(2):153‑61. < PMID: 2918859 > | Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J Clin Pathol. 1993 Apr; 46(4):334‑6. < PMID: 7684403 > | Alvarado M, Klassen C, Cerny J, Horejsi V, Schmidt RE: MEM‑59 monoclonal antibody detects a CD43 epitope involved in lymphocyte activation. Eur J Immunol. 1995 Apr; 25(4):1051‑5. < PMID: 7537669 > | Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland Publishing Inc, New York. 1997; 1‑1342. < NLM ID: 9712219 > | Cermák L, Símová S, Pintzas A, Horejsí V, Andera L: Molecular mechanisms involved in CD43‑mediated apoptosis of TF‑1 cells: Roles of transcription Daxx expression, and adhesion molecules. J Biol Chem. 2002 Mar 8; 277(10):7955‑61. < PMID: 11773067 > | Kadaja L, Laos S, Maimets T: Overexpression of leukocyte marker CD43 causes activation of the tumor suppressor proteins p53 and ARF. Oncogene. 2004 Apr 1; 23(14):2523‑30. < PMID: 14676827 > | Filatov AV, Krotov GI, Zgoda VG, Volkov Y: Fluorescent immunoprecipitation analysis of cell surface proteins: a methodology compatible with mass‑spectrometry. J Immunol Methods. 2007 Jan 30; 319(1‑2):21‑33. < PMID: 17098248 > | Mody PD, Cannon JL, Bandukwala HS, Blaine KM, Schilling AB, Swier K, Sperling AI: Signaling through CD43 regulates CD4 T‑cell trafficking. Blood. 2007 Oct 15; 110(8):2974‑82. < PMID: 17638845 > | Khunkaewla P, Schiller HB, Paster W, Leksa V, Cermák L, Anděra L, Hořejší V, Stockinger H: LFA‑1‑mediated leukocyte adhesion regulated by interaction of CD43 with LFA‑1 and CD147. Mol Immunol. 2007 Nov 8; < PMID: 19203795 > | Simova S, Klima M, Cermak L, Sourkova V, Andera L: Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL‑R1/DR4 to the plasma membrane. Apoptosis. 2008 Mar; 13(3):423‑36. < PMID: 18165900 > | Kadaja-Saarepuu L, Laos S, Jääger K, Viil J, Balikova A, Lõoke M, Hansson GC, Maimets T Skip to main content Skip to navigation Resources How To About NCBI Accesskeys Sign in to NCBI PubMed US National Library of Medicine National Institutes of Health Search databaseSearch termClear inputSearch Create RSSCreate alertAdvancedHelp Result Filters AbstractSend to: Oncogene. 2008 Mar 13;27(12):1705-15. Epub 2007 Sep 24. CD43 promotes cell growth and helps to evade FAS-mediated apoptosis in non-hematopoietic cancer cells lacking the tumor suppressors p53 or ARF. Kadaja-Saarepuu L1, Laos S, Jääger K, Viil J, Balikova A, Lõoke M, Hansson GC, Maimets T.: CD43 promotes cell growth and helps to evade FAS‑mediated apoptosis in non‑hematopoietic cancer cells lacking the tumor suppressors p53 or ARF. Oncogene. 2008 Mar 13; 27(12):1705‑15. < PMID: 17891181 >