CyFlow™ CD34 PerCP-Cy5.5
| Antibody: | Yes |
| Antigen: | CD34 |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | 581 |
| Emission Maximum: | 695 nm |
| Excitation Maximum: | 482 nm |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | PerCP-Cy5.5 |
| Isotype: | IgG1 |
| Laser: | Blue , Green |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Human, Non-Human Primates |
| Product number: | CD394121 |
For Research Use Only
| HLDA Workshop | HLDA V—WS Code MA27 |
| Quantity | 100 tests |
| Volume | 0.4 mL |
| Immunogen | Not available |
| Background Information | CD34 (Mucosialin) is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Janowska-Wieczorek A, Marquez LA, Nabholtz JM, Cabuhat ML, Montaño J, Chang H, Rozmus J, Russell JA, Edwards DR, Turner AR: Growth factors and cytokines upregulate gelatinase expression in bone marrow CD34(+) cells and their transmigration through reconstituted basement membrane. Blood. 1999 May 15; 93(10):3379‑90. < PMID: 10233890 > | Ando K, Nakamura Y, Chargui J, Matsuzawa H, Tsuji T, Kato S, Hotta T: Extensive generation of human cord blood CD34(+) stem cells from Lin(‑)CD34(‑) cells in a long‑term in vitro system. Exp Hematol. 2000 Jun; 28(6):690‑9. < PMID: 10880755 > | Felschow DM, McVeigh ML, Hoehn GT, Civin CI, Fackler MJ: The adapter protein CrkL associates with CD34. Blood. 2001 Jun 15; 97(12):3768‑75. < PMID: 11389015 > | Kato S, Ando K, Nakamura Y, Muguruma Y, Sato T, Yabe H, Yabe M, Hattori K, Yasuda Y, Hotta T: Absence of a CD34‑ hematopoietic precursor population in recipients of CD34+ stem cell transplantation. Bone Marrow Transplant. 2001 Sep; 28(6):587‑95. < PMID: 11607772 > | Suárez L, Vidriales MB, García-Laraña J, Sanz G, Moreno MJ, López A, Barrena S, Martínez R, Tormo M, Palomera L, Lavilla E, López-Berges MC, de Santiago M, de Equiza ME, Miguel JF, Orfao A: CD34+ cells from acute myeloid leukemia, myelodysplastic syndromes, andnormal bone marrow display different apoptosis and drug resistance‑associated phenotypes. Clin Cancer Res. 2004 Nov 15; 10(22):7599‑606. < PMID: 15569991 > | Ono F, Sharma BK, Smith CC, Burnett JW, Aurelian L: CD34+ cells in the peripheral blood transport herpes simplex virus DNA fragments to the skin of patients with erythema multiforme (HAEM).. J Invest Dermatol. 2005 Jun; 124(6):1215‑24. < PMID: 15955097 > | Ninos JM, Jefferies LC, Cogle CR, Kerr WG: The thrombopoietin receptor, c‑Mpl, is a selective surface marker for human hematopoietic stem cells. J Transl Med. 2006 Feb 16; 4:9. < PMID: 16480521 > | Iwasaki H, Kawamoto A, Ishikawa M, Oyamada A, Nakamori S, Nishimura H, Sadamoto K, Horii M, Matsumoto T, Murasawa S, Shibata T, Suehiro S, Asahara T: Dose‑dependent contribution of CD34‑positive cell transplantation to concurrent vasculogenesis and cardiomyogenesis for functional regenerative recovery aftr myocardial infarction. Circulation. 2006 Mar 14; 113(10):1311‑25. < PMID: 16534028 > | Goardon N, Nikolousis E, Sternberg A, Chu WK, Craddock C, Richardson P, Benson R, Drayson M, Standen G, Vyas P, Freeman S: Reduced CD38 expression on CD34+ cells as a diagnostic test in myelodysplastic syndromes. Haematologica. 2009 Aug; 94(8):1160‑3. < PMID: 19644143 > | Sanz E, Muñoz-A N, Monserrat J, Van-Den-Rym A, Escoll P, Ranz I, Alvarez-Mon M, de-la-Hera A: Ordering human CD34+CD10‑CD19+ pre/pro‑B‑cell and CD19‑ common lymphoid progenitor stages in two pro‑B‑cell development pathways. Proc Natl Acad Sci USA. 2010 Mar 30; 107(13):5925‑30. < PMID: 20231472 >