CyFlow™ CD33 Alexa Fluor™ 700
| Alternative Name: | p67 , SIGLECT-3 |
| Antibody: | Yes |
| Antigen: | CD33 |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | WM53 |
| Emission Maximum: | 723 nm |
| Excitation Maximum: | 702 nm |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | Alexa Fluor™ 700 |
| Isotype: | IgG1 |
| Laser: | Red |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Human, Non-Human Primates |
| Product number: | AA248672 |
For Research Use Only
| HLDA Workshop | HLDA IV—WS Code M-505 |
| Quantity | 100 tests |
| Volume | 0.4 mL |
| Immunogen | Human Acute Myelogenous Leukemia (AML) cells |
| Background Information | CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
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