CyFlow™ CD29 Biotin
| Alternative Name: | Integrin β1 |
| Antibody: | Yes |
| Antigen: | CD29 |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | MEM-101A |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | Biotin |
| Isotype: | IgG1 |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Dog, Human, Pig |
| Product number: | BQ324745 |
For Research Use Only
| HLDA Workshop | HLDA VI—WS Code AS A048 |
| Concentration Unit | mg/mL |
| Concentration | 1 |
| Quantity | 0.1 mg |
| Volume | 0.1 mL |
| Immunogen | Raji cells: human Burkitt's lymphoma cell line |
| Background Information | CD29 (β1 integrin subunit, GPIIa) forms non-covalently linked heterodimers with at least 6 different α chains (α1-α6, CD49a-f) determining the binding properties of β1 (VLA) integrins. These integrins mediate cell adhesion to collagen, fibronectin, laminin and other extracellular matrix (ECM) components. This interaction hinders cell death, whereas disruption of anchorage to ECM leads to apoptosis. Decreased expression of most β1 integrins correlates with acquiring multidrug resistance of tumor cells during selection in presence of antitumor drug. In platelets, translocation of intracellular pool of β1 integrins to the plasma membrane following thrombin stimulation. These integrins are also up-regulated in leukocytes during emigration and extravascular migration and appear to be critically involved in regulating the immune cell trafficking from blood to tissue, as well as in regulating tissue damage and disease symptoms related to inflammatory bowel disease. Through a β1 integrin-dependent mechanism, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1β. |
| Usage | The reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working usage is 1·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator. |
| Storage Buffer | The reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Wencel-Drake JD, Dieter MG, Lam SC: Immunolocalization of beta 1 integrins in platelets and platelet‑derived microvesicles. Blood. 1993 Aug 15; 82(4):1197‑203. < PMID: 7688992 > | Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland Publishing Inc, New York. 1997; 1‑1342. < NLM ID: 9712219 > | Werr J, Johansson J, Eriksson EE, Hedqvist P, Ruoslahti E, Lindbom L: Integrin alpha(2)beta(1 (VLA‑2 is a principal receptor used by neutrophils for locomotion in extravascular tissue. Blood. 2000 Mar 1; 95(5):1804‑9. < PMID: 10688841 > | Peng Q, Lai D, Nguyen TT, Chan V, Matsuda T, Hirst SJ: Multiple beta 1 integrins mediate enhancement of human airway smooth muscle cytokine secretion by fibronectin and type I collagen. J Immunol. 2005 Feb 15; 174(4):2258‑64. < PMID: 15699160 > | Lundberg S, Lindholm J, Lindbom L, Hellström PM, Werr J: Integrin alpha2beta1 regulates neutrophil recruitment and inflammatory activity in experimental colitis in mice. Inflamm Bowel Dis. 2006 Mar; 12(3):172‑7. < PMID: 16534417 > | Morozevich GE, Kozlova NI, Preobrazhenskaya ME, Ushakova NA, Eltsov IA, Shtil AA, Berman AE: The role of beta1 integrin subfamily in anchorage‑dependent apoptosis of breast carcinoma cells differing in multidrug resistance. Biochemistry (Mosc). 2006 May; 71(5):489‑95. < PMID: 16732726 > | Simova S, Klima M, Cermak L, Sourkova V, Andera L: Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL‑R1/DR4 to the plasma membrane. Apoptosis. 2008 Mar; 13(3):423‑36. < PMID: 18165900 > | Plánka L, Necas A, Srnec R, Rauser P, Starý D, Jancár J, Amler E, Filová E, Hlucilová J, Kren L, Gál P: Use of allogenic stem cells for the prevention of bone bridge formation in miniature pigs. Physiol Res. 2009; 58(6):885‑93. < PMID: 19093735 > | Machacek C, Supper V, Leksa V, Mitulovic G, Spittler A, Drbal K, Suchanek M, Ohradanova‐Repic A, Stockinger H: Folate Receptor β Regulates Integrin CD11b/CD18 Adhesion of a Macrophage Subset to Collagen. JImmunol. 2016 Sep 15; 197(6):2229‐38. < PMID: 27534550 >