CyFlow™ CD24 APC-Cy7
| Alternative Name: | BA 1 , HAS |
| Antibody: | Yes |
| Antigen: | CD24 |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | SN3 |
| Emission Maximum: | 780 nm |
| Excitation Maximum: | 650 nm, 750 nm |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | APC-Cy7 |
| Isotype: | IgG1 |
| Laser: | Red |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Human |
| Product number: | AG974997 |
For Research Use Only
| HLDA Workshop | HLDA IV—WS Code B 136 HLDA V—WS Code B CD24.7 |
| Quantity | 100 tests |
| Volume | 0.4 mL |
| Immunogen | Glycoproteins purified from human NALM-1 cell line |
| Background Information | CD24 (BA 1, HSA) is a small mucin-like GPI-anchored extracellular membrane glycoprotein expressed on several cell types, including B cells. When B cells are activated and induced to further maturation, however, CD24 begins to disappear. CD24 seems to act as a gate-keeper for lipid rafts, thereby regulating the activity of integrins and other proteins such as the chemokine receptor CXCR4; it is also a ligand for P-selectin. CD24 triggering induces apoptosis of B cell precursors but not in mature resting B cells, where it instead inhibits their ability to proliferate in response to activation. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Barcos M, Pollard C, Fukukawa T, Seon BK: Follicular mantle zone cell subpopulations detected by monoclonal antibody SN3. Hematol Oncol. 1986 Oct-Dec; 4(4):251‐9. < PMID: 3493967 > | Fukukawa T, Matsuzaki H, Haruta Y, Hara H, Seon BK: New monoclonal antibodies SN3, SN3a, and SN3b directed to sialic acid of glycoprotein on human non‑T leukemia cells. Exp Hematol. 1986 Oct; 14(9):850‑5. < PMID: 3489635 > | Knapp W, Dorken B, Gilks W, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (Eds): Leucocyte Typing IV. Oxford University Press, Oxford. 1989; 1‑1820. < NLM ID: 8914679 > | Fischer GF, Majdic O, Gadd S, Knapp W: Signal transduction in lymphocytic and myeloid cells via CD24, a new member of phosphoinositol‑anchored membrane molecules. J Immunol. 1990 Jan 15; 144(2):638‑41. < PMID: 2153173 > | Solvason N, Kearney JF: The human fetal omentum: a site of B cell generation. J Exp Med. 1992 Feb 1; 175(2):397‑404. < PMID: 1370683 > | Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J Clin Pathol. 1993 Apr; 46(4):334‑6. < PMID: 7684403 > | Suzuki T, Kiyokawa N, Taguchi T, Sekino T, Katagiri YU, Fujimoto J: CD24 induces apoptosis in human B cells via the glycolipid‑enriched membrane domains/rafts‑mediated signaling system. J Immunol. 2001 May 1; 166(9):5567‑77. < PMID: 11313396 > | Schabath H, Runz S, Joumaa S, Altevogt P: CD24 affects CXCR4 function in pre‑B lymphocytes and breast carcinoma cells. J Cell Sci. 2006 Jan 15; 119(2):314‑25. < PMID: 16390867 > | Chou YY, Jeng YM, Lee TT, Hu FC, Kao HL, Lin WC, Lai PL, Hu RH, Yuan RH: Cytoplasmic CD24 expression is a novel prognostic factor in diffuse‑type gastri adenocarcinoma. Ann Surg Oncol. 2007 Oct; 14(10):2748‑58. < PMID: 17680316 > | Keller S, Rupp C, Stoeck A, Runz S, Fogel M, Lugert S, Hager HD, Abdel-Bakky MS, Gutwein P, Altevogt P: CD24 is a marker of exosomes secreted into urine and amniotic fluid. Kidney Int. 2007 Nov; 72(9):1095‑102. < PMID: 17700640 > | Runz S, Mierke CT, Joumaa S, Behrens J, Fabry B, Altevogt P: CD24 induces localization of beta1 integrin to lipid raft domains. Biochem Biophys Res Commun. 2008 Jan 4; 365(1):35‑41. < PMID: 17980703 > | Maliar A, Servais C, Waks T, Chmielewski M, Lavy R, Altevogt P, Abken H, Eshhar Z: Redirected T cells that target pancreatic adenocarcinoma antigens eliminate tumors and metastases in mice. Gastroenterology. 2012 Nov; 143(5):1375‑1384. < PMID: 22819865 >