CyFlow™ CD20 PerCP-Cy5.5
| Alternative Name: | B1 , Bp35 |
| Antibody: | Yes |
| Antigen: | CD20 |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | 2H7 |
| Emission Maximum: | 695 nm |
| Excitation Maximum: | 482 nm |
| Field of Interest: | Immunophenotyping, Signaling |
| Format/Fluorochrome: | PerCP-Cy5.5 |
| Isotype: | IgG2b |
| Laser: | Blue , Green |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Human, Non-Human Primates |
| Product number: | AW413198 |
For Research Use Only
| HLDA Workshop | HLDA IV—WS Code B201 |
| Quantity | 100 tests |
| Volume | 0.4 mL |
| Immunogen | Human tonsillar B cells |
| Background Information | CD20 (B1, Bp35) is a cell surface 33-37 (depending on the degree of phosphorylation) kDa non-glycosylated surface phosphoprotein expressed on mature and most malignant B cells, but not stem cells or plasma cells (low number of the CD20 has been also detected on a subpopulation of T lymphocytes and it can be expressed on follicular dendritic cells). Its expression on B cells is synchronous with the expression of surface IgM. CD20 regulates transmembrane calcium conductance (probably functioning as a component of store-operated calcium channel), cell cycle progression and B-cell proliferation. It is associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following mAb treatment. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
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