CyFlow™ CD18 Purified
| Alternative Name: | b2 integrin |
| Antibody: | Yes |
| Antigen: | CD18 |
| Application: | Flow cytometry, Functional assays, Immunoprecipitation, Western blot |
| Clonality: | monoclonal |
| Clone: | MEM-148 |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | Purified |
| Isotype: | IgG1 |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Human |
| Product number: | CW297748 |
For Research Use Only
| Concentration Unit | mg/mL |
| Concentration | 1 |
| Quantity | 0.1 mg |
| Volume | 0.1 mL |
| Immunogen | Peripheral blood mononuclear cells |
| Background Information | CD18 (integrin β2 subunit) forms heterodimers with four types of CD11 molecule to constitute leukocyte (β2) integrins: αLβ2 (CD11a/CD18, LFA-1), αMβ2 (CD11b/CD18, Mac-1, CR3), αXβ2 (CD11c/CD18) and αDβ2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis. |
| Storage Buffer | The reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Gao JX, Issekutz AC: Mac‑1 (CD11b/CD108) is the predominant beta 2 (CD108) integrin mediating human neutrophil migration through synovial and dermal fibroblast barriers. Immunology. 1996 Jul; 88(3):463‑70. < PMID: 8774366 > | Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland Publishing Inc, New York. 1997; 1‑1342. < NLM ID: 9712219 > | Shang XZ, Issekutz AC: Contribution of CD11a/CD18, CD11b/CD18, ICAM‑1 (CD54 and ‑2 (CD102 to human monocyte migration through endothelium and connective tissue fibroblast barriers. Eur J Immunol. 1998 Jun; 28(6):1970‑9. < PMID: 9645379 > | Drbal K, Angelisova P, Cerny J, Pavlistova D, Cebecauer M, Novak P, Horejsi V: Human leukocytes contain a large pool of free forms of CD18. Biochem Biophys Res Commun. 2000 Aug 28; 275(2):295‑9. < PMID: 10964661 > | Drbal K, Angelisova P, Cerny J, Hilgert I, Horejsi V: A novel anti‑CD18 mAb recognizes an activation‑related epitope and induces a high‑affinity conformation in leukocyte integrins. Immunobiology. 2001 May; 203(4):687‑98. < PMID: 11402502 > | Drbal K, Angelisova P, Hilgert I, Cerny J, Novak P, Horejsi V: A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells. Blood. 2001 Sep 1; 98(5):1561‑6. < PMID: 11520808 > | Kess D, Peters T, Zamek J, Wickenhauser C, Tawadros S, Loser K, Varga G, Grabbe S, Nischt R, Sunderkötter C, Müller W, Krieg T, Scharffetter-Kochanek K: CD4+ T cell‑associated pathophysiology critically depends on CD18 gene dose effects in a murine model of psoriasis. J Immunol. 2003 Dec 1; 171(11):5697‑706. < PMID: 14634077 > | Solovjov DA, Pluskota E, Plow EF: Distinct roles for the alpha and beta subunits in the functions of integrin alphaMbeta2. J Biol Chem. 2005 Jan 14; 280(2):1336‑45. < PMID: 15485828 > | Peters T, Sindrilaru A, Wang H, Oreshkova T, Renkl AC, Kess D, Scharffetter-Kochanek K: CD18 in monogenic and polygenic inflammatory processes of the skin. J Investig Dermatol Symp Proc. 2006 Sep; 11(1):42186. < PMID: 17069006 > | Dassanayake RP, Maheswaran SK, Srikumaran S: Monomeric expression of bovine beta2‑integrin subunits reveals their role in Mannheimia haemolytica leukotoxin‑induced biological effects. Infect Immun. 2007 Oct; 75(10):5004‑10. < PMID: 17698568 > | Aubert M, Yoon M, Sloan DD, Spear PG, Jerome KR: The virological synapse facilitates herpes simplex virus entry into T cells. J Virol. 2009 Jun; 83(12):6171‑83. < PMID: 19339346 > | Osicka R, Osickova A, Hasan S, Bumba L, Cerny J, Sebo P: Adenylate cyclase toxin is a unique ligand of the integrin complement receptor 3. eLife. 2015 Dec 9; 4:e10766. < PMID: 26650353 > | Kanderova V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, Hrusak O, Lund‐Johansen F, Kalina T: High‐resolution antibody array analysis of childhood acute leukemia cells. Mol Cell Proteomics. 2016 Apr 1; 15(4):1246‐61. < PMID: 26785729 > | Machacek C, Supper V, Leksa V, Mitulovic G, Spittler A, Drbal K, Suchanek M, Ohradanova‐Repic A, Stockinger H: Folate Receptor β Regulates Integrin CD11b/CD18 Adhesion of a Macrophage Subset to Collagen. JImmunol. 2016 Sep 15; 197(6):2229‐38. < PMID: 27534550 >