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CyFlow™ CD13 Alexa Fluor™ 488

CyFlow™ CD13 Alexa Fluor™ 488
Alternative Name: APN , gp150
Antibody: Yes
Antigen: CD13
Application: Flow cytometry
Clonality: monoclonal
Clone: WM15
Emission Maximum: 519 nm
Excitation Maximum: 495 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: Alexa Fluor™ 488
Isotype: IgG1
Laser: Blue
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human, Non-Human Primates
Product number: CR471166

For Research Use Only

$285.00 USD*

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Description Documentation TDS & SDS
Description
HLDA Workshop HLDA III—WS Code M 213 HLDA IV—WS Code M 44 HLDA IV—WS Code M 209 HLDA V—WS... more
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CyFlow™ CD13 Alexa Fluor™ 488
HLDA WorkshopHLDA III—WS Code M 213 HLDA IV—WS Code M 44 HLDA IV—WS Code M 209 HLDA V—WS Code M MA191
Quantity100 tests
Volume0.4 mL
ImmunogenHuman Acute Myelogenous Leukemia (AML) cells
Background InformationCD13 (APN; aminopeptidase N) is a 150 kDa type II transmembrane zinc-binding ectopeptidase expressed on various cell types. This metalloprotease preferentially catalyzes removal of neutral amino acids from small peptides, thus activating or inactivating bioactive peptides. CD13 has also role in extracellular matrix degradation, antigen processing and signal transduction, is important in inflammatory responses, regulates intercellular contact, cell motility and vascularization. CD13 is involved in protection of leukemic cells against apoptosis and its expression associated with poor prognosis of carcinomas.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Bradstock KF, Favaloro EJ, Kabral A, Kerr A, Hughes WG, Berndt MC, Musgrove E: Human myeloid differentiation antigens identified by monoclonal antibodies: expression on leukemic cells. Pathology. 1985 Jul; 17(3):392‑9. < PMID: 3865147 > | Bradstock KF, Favaloro EJ, Kabral A, Kerr A, Hughes WG, Musgrove E: Myeloid progenitor surface antigen identified by monoclonal antibody. Br J Haematol. 1985 Sep; 61(1):44136. < PMID: 4052321 > | McMichael AJ, Beverley PCL, Cobbold S, et al (Eds): Leucocyte Typing III, White Cell Differentiation Antigens. Oxford University Press, Oxford. 1987; 1‑1050. < NLM ID: 8913266 > | Knapp W, Dorken B, Gilks W, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (Eds): Leucocyte Typing IV. Oxford University Press, Oxford. 1989; 1‑1820. < NLM ID: 8914679 > | Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J Clin Pathol. 1993 Apr; 46(4):334‑6. < PMID: 7684403 > | Favaloro EJ, Browning T, Facey D: CD13 (GP150: aminopeptidase‑N): predominant functional activity in blood is localized to plasma and is not cell‑surface associated. Exp Hematol. 1993 Dec; 21(13):1695‑701. < PMID: 7902291 > | Tokuhara T, Hattori N, Ishida H, Hirai T, Higashiyama M, Kodama K, Miyake M: Clinical significance of aminopeptidase N in non‑small cell lung cancer. Clin Cancer Res. 2006 Jul 1; 12(13):3971‑8. < PMID: 16818694 > | Petrovic N, Schacke W, Gahagan JR, O'Conor CA, Winnicka B, Conway RE, Mina-Osorio P, Shapiro LH: CD13/APN regulates endothelial invasion and filopodia formation. Blood. 2007 Jul 1; 110(1):142‑50. < PMID: 17363739 > | Terauchi M, Kajiyama H, Shibata K, Ino K, Nawa A, Mizutani S, Kikkawa F: Inhibition of APN/CD13 leads to suppressed progressive potential in ovarian carcinoma cells. BMC Cancer. 2007 Jul 27; 7:140. < PMID: 17655775 > | McCormack E, Mujić M, Osdal T, Bruserud Ø , Gjertsen BT: Multiplexed mAbs: a new strategy in preclinical time‑domain imaging of acute myeloid leukemia. Blood. 2013 Feb 14; 121(7):e34‑42. < PMID: 23243270 >

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