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CyFlow™ CD8b Purified

CyFlow™ CD8b Purified
Antibody: Yes
Antigen: CD8b
Application: Flow cytometry, Functional assays, Immunohistochemistry (frozen sections), Immunoprecipitation, Western blot
Clonality: monoclonal
Clone: 341
Field of Interest: Immunophenotyping, MHC
Format/Fluorochrome: Purified
Isotype: IgG1
Regulatory Status: RUO
Source Species: Mouse
Target Species: Rat
Product number: BP764082

For Research Use Only

$120.00 USD*

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Description
Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL... more
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CyFlow™ CD8b Purified
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenCD8 positive Wistar rat splenic T cell hybridomas
Background InformationCD8b (CD8 β) subunit of CD8 T cell coreceptor is expressed in CD8 α/β heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 α/α homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages, mast cells, and dendritic cells. Regulation of CD8 β level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 α/β but not α/α dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Torres-Nagel N, Kraus E, Brown MH, Tiefenthaler G, Mitnacht R, Williams AF, Hünig T: Differential thymus dependence of rat CD8 isoform expression. Eur J Immunol. 1992 Nov; 22(11):2841‑8. < PMID: 1358623 > | Kühnlein P, Park JH, Herrmann T, Elbe A, Hünig T: Identification and characterization of rat gamma/delta T lymphocytes in peripheral lymphoid organs, small intestine, and skin with a monoclonal antibody to a constant determinant of the gamma/delta T cell receptor. J Immunol. 1994 Aug1; 153(3):979‑86. < PMID: 8027567 > | Kraus E, Lambracht D, Wonigeit K, Hünig T: Negative regulation of rat natural killer cell activity by major histocompatibility complex class I recognition. Eur J Immunol. 1996 Nov; 26(11):2582‑6. < PMID: 8921942 > | Hirji N, Lin TJ, Befus AD: A novel CD8 molecule expressed by alveolar and peritoneal macrophages stimulates nitric oxide production. J Immunol. 1997 Feb 15; 158(4):1833‑40. < PMID: 9029123 > | Hirji N, Lin TJ, Bissonnette E, Belosevic M, Befus AD: Mechanisms of macrophage stimulation through CD8: macrophage CD8alpha and CD8beta induce nitric oxide production and associated killing of the parasite Leishmania major. J Immunol. 1998 Jun 15; 160(12):6004‑11. < PMID: 9637515 > | Lin TJ, Hirji N, Nohara O, Stenton GR, Gilchrist M, Befus AD: Mast cells express novel CD8 molecules that selectively modulate mediator secretion. J Immunol. 1998 Dec 1; 161(11):6265‑72. < PMID: 9834115 > | Ellerman KE, Like AA: Islet cell membrane antigens activate diabetogenic CD4+ T‑cells in the BB/Wor rat. Diabetes. 1999 May; 48(5):975‑82. < PMID: 10331400 > | Nohara O, Kulka M, Déry RE, Wills FL, Hirji NS, Gilchrist M, Befus AD: Regulation of CD8 expression in mast cells by exogenous or endogenous nitric oxide. J Immunol. 2001 Nov 15; 167(10):5935‑9. < PMID: 11698471 > | Mabarrack NH, Turner NL, Mayrhofer G: Recent thymic origin, differentiation, and turnover of regulatory T cells. J Leukoc Biol. 2008 Nov; 84(5):1287‑97. < PMID: 18682578 >

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