CyFlow™ CD4 PE-Cy7
Alternative Name: | Leu3a, T4 |
Antibody: | Yes |
Antigen: | CD4 |
Application: | Flow cytometry |
Clonality: | monoclonal |
Clone: | MEM-241 |
Emission Maximum: | 785 nm |
Excitation Maximum: | 496 nm, 565 nm |
Field of Interest: | Immunophenotyping |
Format/Fluorochrome: | PE-Cy7 |
Isotype: | IgG1 |
Laser: | Blue , Green, Yellow |
Regulatory Status: | RUO |
Source Species: | Mouse |
Target Species: | Human |
Product number: | AQ743991 |
For Research Use Only
HLDA Workshop | HCDM (HLDA VIII)—WS Code M241 |
Quantity | 100 tests |
Volume | 0.4 mL |
Immunogen | 2 N-terminal domains of human CD4 fused to human IgG1 Fc |
Background Information | CD4 (T4, Leu3a) is a 55 kDa single-chain transmembrane glycoprotein expressed on a subset of T lymphocytes (''helper'' T-cells) and also on monocytes, tissue macrophages and granulocytes. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1, 2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. |
Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
Stability | Do not use after expiration date stamped on vial label. |
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