CyFlow™ CD4 PE-Cy5
| Alternative Name: | Leu3a, T4 |
| Antibody: | Yes |
| Antigen: | CD4 |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | MEM-241 |
| Emission Maximum: | 670 nm |
| Excitation Maximum: | 496 nm, 565 nm |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | PE-Cy5 |
| Isotype: | IgG1 |
| Laser: | Blue , Green, Yellow |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Human |
| Product number: | BF866489 |
For Research Use Only
| HLDA Workshop | HCDM (HLDA VIII)—WS Code M241 |
| Quantity | 100 tests |
| Volume | 0.4 mL |
| Immunogen | 2 N-terminal domains of human CD4 fused to human IgG1 Fc |
| Background Information | CD4 (T4, Leu3a) is a 55 kDa single-chain transmembrane glycoprotein expressed on a subset of T lymphocytes (''helper'' T-cells) and also on monocytes, tissue macrophages and granulocytes. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1, 2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
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