CyFlow™ Beta3-tubulin FITC

CyFlow™ Beta3-tubulin FITC
Antigen: β3-tubulin
Application: Immunocytochemistry
Clonality: monoclonal
Clone: TU-20
Emission Maximum: 518 nm
Excitation Maximum: 490 to 495 nm
Field of Interest: Cytoskeleton, Neurobiology
Format/Fluorochrome: FITC
Isotype: IgG1
Laser: Blue
Regulatory Status: RUO
Source Species: Mouse
Target Species: Broad Spectrum
Product number: AR041515

For Research Use Only

$235.00 USD*

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Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL... more
CyFlow™ Beta3-tubulin FITC
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenPeptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide
Background Informationβ3-tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumors originating from these cells. The β3-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.
UsageThe reagent is designed for Immunocytochemistry. Suggested working usage is 2.5·µg/ml. The conjugate was also successfully used on paraffin sections using confocal microscopy. Working concentrations should be determined by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Draberova E, Lukas Z, Ivanyi D, Viklicky V, Draber P: Expression of class III beta‑tubulin in normal and neoplastic human tissues.. Histochem·Cell·Biol. 1998·Mar; 109(3):231‑9. <·PMID:·9541471·> | Peknicova J, Kubatova A, Sulimenko V, Draberova E, Viklicky V, Hozak P, Draber P: Differential subcellular distribution of tubulin epitopes in boar spermatozoa:recognition of class III beta‑tubulin epitope in sperm tail. Biol·Reprod. 2001·Sep; 65(3):672‑9. <·PMID:·11514327·> | Kukharskyy V, Sulimenko V, Macůrek L, Sulimenko T, Dráberová E, Dráber P: Complexes of gamma‑tubulin with nonreceptor protein tyrosine kinases Src and Fynin differentiating P19 embryonal carcinoma cells. Exp·Cell·Res. 2004·Aug·1; 298(1):218‑28. <·PMID:·15242776·> | Jirasek T, Pisarikova E, Viklicky V, Mandys V: Expression of class III beta‑tubulin in malignant epithelial tumours: an immunohistochemical study using TU‑20 and TuJ‑1 antibodies. Folia·Histochem·Cytobiol. 2007; 45(1):41‑5. <·PMID:·17378245·> | Katsetos CD, Dráberová E, Smejkalová B, Reddy G, Bertrand L, de Chadarévian J-P, Legido A, Nissanov J, Baas PW, Dráber P: Class III beta‑tubulin and gamma‑tubulin are co‑expressed and form complexes in human glioblastoma cells.. Neurochem·Res. 2007; 32:1387‑1398. <·PMID:·20162618·> | Jirásek T, Cipro S, Musilová A, Kubecová M, Mandys V: Expression of class III beta‑tubulin in colorectal carcinomas: an immunohistochemical study using TU‑20&TuJ‑1 antibody. Indian·J·Med·Res. 2009·Jan; 129(1):89‑94. <·PMID:·19287064·> | Theodorou E, Dalembert G, Heffelfinger C, White E, Weissman S, Corcoran L, Snyder M: A high throughput embryonic stem cell screen identifies Oct‑2 as a bifunctional regulator of neuronal differentiation. Genes·Dev. 2009·Mar·1; 23(5):575‑88. <·PMID:·19270158·> | Dráberová E, Sulimenko V, Vinopal S, Sulimenko T, Sládková V, D'Agostino L, Sobol M, Hozák P, Křen L, Katsetos CD, Dráber P: Differential expression of human γ‐tubulin isotypes during neuronal development and oxidative stress points to a γ‐tubulin‐2 prosurvival function. FASEBJ. 2017 May; 31(5):1828‐1846. <·PMID: 28119396·>

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