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CyFlow™ IgG2b PE-Cy5

CyFlow™ IgG2b PE-Cy5
Antibody: Yes
Antigen: IgG2b Isotype Control
Application: Flow cytometry (Isotype control)
Clonality: monoclonal
Clone: MPC-11
Emission Maximum: 670 nm
Excitation Maximum: 496 nm, 565 nm
Field of Interest: Isotype controls
Format/Fluorochrome: PE-Cy5
Isotype: IgG2b
Laser: Blue , Green, Yellow
Regulatory Status: RUO
Source Species: Mouse
Product number: CV731219

For Research Use Only

$285.00 USD*

Price excludes any applicable taxes plus shipping costs

Description Documentation TDS & SDS
Description
Concentration Unit mg/mL Concentration 0,1 Quantity 0.1 mg Volume 1.0 mL... more
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CyFlow™ IgG2b PE-Cy5

Concentration Unitmg/mL
Concentration0,1
Quantity0.1 mg
Volume1.0 mL
ImmunogenKLH-coupled trinitrophenol
Background InformationThe specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μ/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MPC-11 was generated against an epitope irrelevant for human, mouse, and rat material, and can thus be used for evaluation of the background staining that is caused by general nonspecific interactions between an mouse IgG2b molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
UsageThe reagent is designed as an isotype control for Flow Cytometry analysis. To establish the amount of non-specific antibody binding, match the concentration of the correct isotype to the recommended working concentration of the antigen-specific antibody. If the background signal of the isotype control is too high (usually when working antibody concentrations are above 10·µg/ml of incubation mixture), change the experimental conditions to reduce the background.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Gupta D, Wang Q, Vinson C, Dziarski R: Bacterial peptidoglycan induces CD14‑dependent activation of transcription factors CREB/ATF and AP‑1. J Biol Chem. 1999 May 14; 274(20):14012‑20. < PMID: 10318814 > | Im JS, Tapinos N, Chae GT, Illarionov PA, Besra GS, DeVries GH, Modlin RL, Sieling PA, Rambukkana A, Porcelli SA: Expression of CD1d molecules by human schwann cells and potential interactions with immunoregulatory invariant NK T cells. J Immunol. 2006 Oct 15; 177(8):5226‑35. < PMID: 17015708 > | Smed-Sörensen A, Moll M, Cheng TY, Loré K, Norlin AC, Perbeck L, Moody DB, Spetz AL, Sandberg JK: IgG regulates the CD1 expression profile and lipid antigen‑presenting function in human dendritic cells via FcgammaRIIa. Blood. 2008 May 15; 111(10):5037‑46. < PMID: 18337560 > | Liang XS, Zhou Y, Li CZ, Wan MB: Natural course of chronic hepatitis B is characterized by changing patterns of programmed death type‑1 of CD8‑positive T cells. World J Gastroenterol. 2010 Feb 7; 16(5):618‑24. < PMID: 20128032 > | Dewals BG, Vanderplasschen A: Malignant catarrhal fever induced by Alcelaphine herpesvirus 1 is characterized by an expansion of activated CD3+CD8+CD4‑ T cells expressing a cytotoxic phenotype in both lymphoid and non‑lymphoid tissues.. Vet Res. 2011 Aug 22; 42:95. < PMID: 21859474 >

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