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CyFlow™ TCR alpha/beta Purified

CyFlow™ TCR alpha/beta Purified
Antibody: Yes
Antigen: TCR α/β
Application: Flow cytometry, Functional assays, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western blot
Clonality: monoclonal
Clone: R73
Field of Interest: Immunophenotyping, MHC
Format/Fluorochrome: Purified
Isotype: IgG1
Regulatory Status: RUO
Source Species: Mouse
Target Species: Non-Human Primates, Rat
Product number: CH797281

For Research Use Only

$120.00 USD*

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Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL... more
CyFlow™ TCR alpha/beta Purified
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenRat T blasts and erythrocytes
Background InformationThe antigen-specific T cell receptor (TCR) is composed of either α and β subunit, or γ and δ subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR α/β heterodimers, whereas the T cells expressing TCR γ/δ heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Hünig T, Wallny HJ, Hartley JK, Lawetzky A, Tiefenthaler G: A monoclonal antibody to a constant determinant of the rat T cell antigen receptor that induces T cell activation: Differential reactivity with subsets of immature and mature T lymphocytes. J Exp Med. 1989 Jan 1; 169(1):73‑86. < PMID: 2783336 > | Kraus E, Lambracht D, Wonigeit K, Hünig T: Negative regulation of rat natural killer cell activity by major histocompatibility complex class I recognition. Eur J Immunol. 1996 Nov; 26(11):2582‑6. < PMID: 8921942 > | Hansson AS, Heinegard D, Holmdahl R: A new animal model for relapsing polychondritis, induced by cartilage matrix protein (matrilin‑1). J Clin Invest. 1999 Sep; 104(5):589‑98. < PMID: 10487773 > | Carlson BC, Jansson AM, Larsson A, Bucht A, Lorentzen JC: The endogenous adjuvant squalene can induce a chronic T‑cell‑mediated arthritis in rats. Am J Pathol. 2000 Jun; 156(6):2057‑65. < PMID: 10854227 > | Ritter T, Schröder G, Risch K, Vergopoulos A, Shean MK, Kolls J, Brock J, Lehmann M, Volk HD: Ischemia/reperfusion injury‑mediated down‑regulation of adenovirus‑mediated gene expression in a rat heart transplantation model is inhibited by co‑application of a TNFRp55‑Ig chimeric construct. Gene Ther. 2000 Jul; 7(14):1238‑43. < PMID: 10918493 > | Wilhelm MJ, Pratschke J, Beato F, Taal M, Kusaka M, Hancock WW, Tilney NL: Activation of the heart by donor brain death accelerates acute rejection after transplantation. Circulation. 2000 Nov 7; 102(19):2426‑33. < PMID: 11067799 > | Li S, Thanikachalam M, Pang M, Kawaharada N, Aitouche A, Pham SM: A clinically relevant CTLA4‑Ig‑based regimen induces chimerism and tolerance to heart grafts. Ann Thorac Surg. 2001 Oct; 72(4):1306‑10. < PMID: 11603451 > | Kerstan A, Hünig T: Cutting edge: distinct TCR‑ and CD28‑derived signals regulate CD95L, Bcl‑xL, and the survival of primary T cells. J Immunol. 2004 Feb 1; 172(3):1341‑5. < PMID: 14734708 > | Kreiss M, Asmuss A, Krejci K, Lindemann D, Miyoshi-Akiyama T, Uchiyama T, Rink L, Broeren CP, Herrmann T: Contrasting contributions of complementarity‑determining region 2 and hypervariable region 4 of rat BV8S2+ (Vbeta8.2) TCR to the recognition of myelin basic protein and different types of bacterial superantigens. Int Immunol. 2004 May; 16(5):655‑63. < PMID: 15096488 > | Shao H, Sun SL, Kaplan HJ, Sun D: Characterization of rat CD8+ uveitogenic T cells specific for interphotoreceptor retinal‑binding protein 1177‑1191. J Immunol. 2004 Aug 15; 173(4):2849‑54. < PMID: 15295004 > | Radojevic K, Arsenovic-Ranin N, Kosec D, Pesic V, Pilipovic I, Perisic M, Plecas-Solarovic B, Leposavic G: Neonatal castration affects intrathymic kinetics of T‑cell differentiation and the spleen T‑cell level. J Endocrinol. 2007 Mar; 192(3):669‑82. < PMID: 17332534 > | Müller N, van den Brandt J, Odoardi F, Tischner D, Herath J, Flügel A, Reichardt HM: A CD28 superagonistic antibody elicits 2 functionally distinct waves of T cell activation in rats. J Clin Invest. 2008 Apr; 118(4):1405‑16. < PMID: 18357346 > | Fuller JM, Bogdani M, Tupling TD, Jensen RA, Pefley R, Manavi S, Cort L, Blankenhorn EP, Mordes JP, Lernmark A, Kwitek AE: Genetic dissection reveals diabetes loci proximal to the gimap5 lymphopenia gene. Physiol Genomics. 2009 Jun 10; 38(1):89‑97. < PMID: 19351909 >