CyFlow™ CD33 PE

CyFlow™ CD33 PE
Laser: Blue, Green, Yellow
Target Species: Human, Non-Human Primates
Antigen: CD33
Field of Interest: Immunophenotyping
Clone: WM53
Regulatory Status: RUO
Alternative Name: p67, SIGLECT-3
Application: Flow cytometry
Format/Fluorochrome: PE
Species of Origin: Mouse
Clonality: monoclonal
Emission Maximum: 576 nm
Excitation Maximum: 496 nm, 565 nm
Isotype: IgG1
Product number: BW164790

For Research Use Only

$255.00 USD*

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HLDA Workshop HLDA IV—WS Code M-505 Quantity 100 tests Immunogen Human AML cells... more
CyFlow™ CD33 PE
HLDA WorkshopHLDA IV—WS Code M-505
Quantity100 tests
ImmunogenHuman AML cells
SpecifityThe mouse monoclonal antibody WM53 recognize CD33 antigen, a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells; it is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.
Background InformationCD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.1% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Favaloro EJ, Bradstock KF, Kabral A, Grimsley P, Berndt MC: Characterization of monoclonal antibodies to the human myeloid‑differentiation antigen, 'gp67' (CD‑33). Dis·Markers. 1987·Dec; 5(4):215‑25. <·PMID:·3502990·> | Favaloro EJ, Bradstock KF, Kabral A, Grimsley P, Zowtyj H, Zola H: Further characterization of human myeloid antigens (gp160, 95, gp150, gp67) investigation of epitopic heterogeneity and non‑haemopoietic distributionusing panels of monoclonal antibodies belonging to CD‑11b, CD‑13 and CD‑33. Br·J·Haematol. 1988·Jun; 69(2):163‑71. <·PMID:·2455535·> | Bradstock KF, Kirk J, Grimsley PG, Kabral A, Hughes WG: Unusual immunophenotypes in acute leukaemias: incidence and clinical correlations. Br·J·Haematol. 1989·Aug; 72(4):512‑8. <·PMID:·2673329·> | Shin YK, Choi EY, Kim SH, Chung J, Chung DH, Park WS, Jung KC, Kim HS, Park S, Kim HJ, Park MH, Min CK, Kim CC, Park SH: Expression of leukemia‑associated antigen, JL1, in bone marrow and thymus. Am·J·Pathol. 2001·Apr; 158(4):1473‑80. <·PMID:·11290565·> | Vitale C, Romagnani C, Puccetti A, Olive D, Costello R, Chiossone L, Pitto A, Bacigalupo A, Moretta L, Mingari MC: Surface expression and function of p75/AIRM‑1 or CD33 in acute myeloid leukemias: engagement of CD33 induces apoptosis of leukemic cells. Proc·Natl·Acad·Sci·USA. 2001·May·8; 98(10):5764‑9. <·PMID:·11320212·> | Hernández-López C, Varas A, Sacedón R, Jiménez E, Muñoz JJ, Zapata AG, Vicente A: Stromal cell‑derived factor 1/CXCR4 signaling is critical for early human T‑cell development. Blood. 2002·Jan·15; 99(2):546‑54. <·PMID:·11781237·> | Schenk M, Bouchon A, Birrer S, Colonna M, Mueller C: Macrophages expressing triggering receptor expressed on myeloid cells‑1 are underrepresented in the human intestine. J·Immunol. 2005·Jan·1; 174(1):517‑24. <·PMID:·15611278·> | Garnache-Ottou F, Chaperot L, Biichle S, Ferrand C, Remy-Martin JP, Deconinck E, de Tailly PD, Bulabois B, Poulet J, Kuhlein E, Jacob MC, Salaun V, Arock M, Drenou B, Schillinger F, Seilles E, Tiberghien P, Bensa JC, Plumas J, Saas P: Expression of the myeloid‑associated marker CD33 isnot an exclusive factor for leukemic plasmacytoid dendritic cells. Blood. 2005·Feb·1; 105(3):1256‑64. <·PMID:·15388576·> | Leone AM, Rutella S, Bonanno G, Abbate A, Rebuzzi AG, Giovannini S, Lombardi M, Galiuto L, Liuzzo G, Andreotti F, Lanza GA, Contemi AM, Leone G, Crea F: Mobilization of bone marrow‑derived stem cells after myocardial infarction and left ventricular function. Eur·Heart·J. 2005·Jun; 26(12):1196‑204. <·PMID:·15734770·> | Hernández-Caselles T, Martínez-Esparza M, Pérez-Oliva AB, Quintanilla-Cecconi AM, García-Alonso A, Alvarez-López DM, García-Peñarrubia P: A study of CD33 (SIGLEC‑3) antigen expression and function on activated human T and NK cells: two isoforms of CD33 are generated by alternative splicing. J·Leukoc·Biol. 2006·Jan; 79(1):46‑58. <·PMID:·16380601·>

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