CyFlow™ CD28 Pacific Blue™

CyFlow™ CD28 Pacific Blue™
Laser: Violet
Target Species: Human, Non-Human Primates
Antigen: CD28
Field of Interest: Immunophenotyping
Clone: CD28.2
Regulatory Status: RUO
Alternative Name: Tp44
Application: Flow cytometry
Format/Fluorochrome: Pacific Blue™
Species of Origin: Mouse
Clonality: monoclonal
Emission Maximum: 455 nm
Excitation Maximum: 400 to 410 nm
Isotype: IgG1
Product number: BS327469

For Research Use Only

$290.00 USD*

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Quantity 100 tests Immunogen DC28.1.3.3 murine T cell hybridoma transfected with human... more
CyFlow™ CD28 Pacific Blue™
Quantity100 tests
ImmunogenDC28.1.3.3 murine T cell hybridoma transfected with human CD28 cDNA
SpecifityThe mouse monoclonal antibody CD28.2 recognizes CD28 antigen, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.
Background InformationCD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.1% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Nunès J, Klasen S, Ragueneau M, Pavon C, Couez D, Mawas C, Bagnasco M, Olive D: CD28 mAbs with distinct binding properties differ in their ability to induce T cell activation: analysis of early and late activation events. Int·Immunol. 1993·Mar; 5(3):311‑5. <·PMID:·8385476·> | Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J·Clin·Pathol. 1993·Apr; 46(4):334‑6. <·PMID:·7684403·> | Nunes J, Klasen S, Franco MD, Lipcey C, Mawas C, Bagnasco M, Olive D: Signalling through CD28 T‑cell activation pathway involves an inosito phospholipid‑specific phospholipase C activity. Biochem·J. 1993·Aug·1; 293 (3):835‑42. <·PMID:·8394695·> | Galea-Lauri J, Darling D, Gan SU, Krivochtchapov L, Kuiper M, Gäken J, Souberbielle B, Farzaneh F: Expression of a variant of CD28 on a subpopulation of human NK cells: implications for B7‑mediated stimulation of NK cells. J·Immunol. 1999·Jul·1; 163(1):62‑7. <·PMID:·10384130·> | Tazi A, Moreau J, Bergeron A, Dominique S, Hance AJ, Soler P: Evidence that Langerhans cells in adult pulmonary Langerhans cell histiocytosis are mature dendritic cells: importance of the cytokine microenvironment. J·Immunol. 1999·Sep·15; 163(6):3511‑5. <·PMID:·10477625·> | Marti F, Krause A, Post NH, Lyddane C, Dupont B, Sadelain M, King PD: Negative‑feedback regulation of CD28 costimulation by a novel mitogen‑activated protein kinase phosphatase, MKP6. J·Immunol. 2001·Jan·1; 166(1):197‑206. <·PMID:·11123293·> | Scharschmidt E, Wegener E, Heissmeyer V, Rao A, Krappmann D: Degradation of Bcl10 induced by T‑cell activation negatively regulates NF‑kappa B signaling. Mol·Cell·Biol. 2004·May; 24(9):3860‑73. <·PMID:·15082780·> | Jeong SH, Qiao M, Nascimbeni M, Hu Z, Rehermann B, Murthy K, Liang TJ: Immunization with hepatitis C virus‑like particles induces humoral and cellular immune responses in nonhuman primates. J·Virol. 2004·Jul; 78(13):6995‑7003. <·PMID:·15194776·>

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