CyFlow™ CD22 Azide Free

CyFlow™ CD22 Azide Free
Target Species: Human
Antigen: CD22
Field of Interest: Immunophenotyping
Clone: IS7
Regulatory Status: RUO
Alternative Name: BL-CAM, SIGLEC-2, SIGLEC2
Application: Flow cytometry
Format/Fluorochrome: Azide Free
Species of Origin: Mouse
Clonality: monoclonal
Isotype: IgG1
Product number: CL011891

For Research Use Only

$120.00 USD*

Price excludes any applicable taxes plus shipping costs

Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Immunogen Reh human cell... more
CyFlow™ CD22 Azide Free
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
ImmunogenReh human cell line
SpecifityThe mouse monoclonal antibody IS7 recognizes CD22 antigen, a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes; it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes.
Background InformationCD22 (Siglec-2; sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding α 2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.
Storage BufferThe reagent is provided in azide-free phosphate buffered saline (PBS) solution, pH ≈7.4; 0.2 µm filter sterilized.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Knapp W, Dorken B, Gilks W, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (Eds): Leucocyte Typing IV. Oxford·University·Press,·Oxford. 1989; 1‑1820. <·NLM·ID:·8914679·> | Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J·Clin·Pathol. 1993·Apr; 46(4):334‑6. <·PMID:·7684403·> | Tedder TF, Poe JC, Haas KM: CD22: A Multifunctional Receptor That Regulates B Lymphocyte Survival and Signal Transduction. Adv·Immunol. 2005; 88:1‑50. <·PMID:·16227086·> | Tateno H, Li H, Schur MJ, Bovin N, Crocker PR, Wakarchuk WW, Paulson JC: Distinct endocytic mechanisms of CD22 (Siglec‑2 and Siglec‑F reflect roles in cell signaling and innate immunity. Mol·Cell·Biol. 2007·Aug; 27(16):5699‑710. <·PMID:·17562860·> | Walker JA, Smith KG: CD22: An inhibitory enigma. Immunology. 2008·Mar; 123(3):314‑25. <·PMID:·18067554·>

Article successfully added.