CyFlow™ CD19 Purified

CyFlow™ CD19 Purified
Target Species: Mouse
Antigen: CD19
Field of Interest: Immunophenotyping, MHC
Clone: 1D3
Regulatory Status: RUO
Alternative Name: B4
Application: Flow cytometry, Functional assays, Immunohistochemistry (frozen sections), Immunoprecipitation
Format/Fluorochrome: Purified
Species of Origin: Rat
Clonality: monoclonal
Isotype: IgG2a
Product number: CG704425

For Research Use Only

$120.00 USD*

Price excludes any applicable taxes plus shipping costs

Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Immunogen Mouse... more
CyFlow™ CD19 Purified
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
ImmunogenMouse CD19-transfected cell line
SpecifityThe rat monoclonal antibody 1D3 recognizes mouse CD19 antigen, 95 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on B lymphocytes and follicular dendritic cells; it is lost on plasma cells.
Background InformationCD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.1% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Krop I, de Fougerolles AR, Hardy RR, Allison M, Schlissel MS, Fearon DT: Self‑renewal of B‑1 lymphocytes is dependent on CD19. Eur·J·Immunol. 1996·Jan; 26(1):238‑42. <·PMID:·8566073·> | Krop I, Shaffer AL, Fearon DT, Schlissel MS: The signaling activity of murine CD19 is regulated during cell development. J·Immunol. 1996·Jul·1; 157(1):48‑56. <·PMID:·8683154·> | Cherukuri A, Cheng PC, Pierce SK: The role of the CD19/CD21 complex in B cell processing and presentation of complement‑tagged antigens. J·Immunol. 2001·Jul·1; 167(1):163‑72. <·PMID:·11418645·> | Inabe K, Kurosaki T: Tyrosine phosphorylation of B‑cell adaptor for phosphoinositide 3‑kinase is required for Akt activation in response to CD19 engagement. Blood. 2002·Jan·15; 99(2):584‑9. <·PMID:·11781242·> | Shoham T, Rajapaksa R, Boucheix C, Rubinstein E, Poe JC, Tedder TF, Levy S: The tetraspanin CD81 regulates the expression of CD19 during B cell development in a postendoplasmic reticulum compartment. J·Immunol. 2003·Oct·15; 171(8):4062‑72. <·PMID:·14530327·>

Article successfully added.