CyFlow™ CD16 Biotin

CyFlow™ CD16 Biotin
Target Species: Human
Antigen: CD16
Field of Interest: Immunophenotyping
Clone: MEM-154
Regulatory Status: RUO
Alternative Name: FcγRIII
Application: Flow cytometry
Format/Fluorochrome: Biotin
Species of Origin: Mouse
Clonality: monoclonal
Isotype: IgG1
Product number: AE412174

For Research Use Only

$160.00 USD*

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Concentration 1 mg/mL Quantity 0.1 mg Volume 0.1 mL Immunogen Human granulocytes... more
CyFlow™ CD16 Biotin
Concentration1 mg/mL
Quantity0.1 mg
Volume0.1 mL
ImmunogenHuman granulocytes
Background InformationCD16 (FcγRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcγRIII is expressed in two forms - FcγRIIIA and FcγRIIIB. FcγRIIIA is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcεRI-γ subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcγRIIIA is associated, moreover, with FcεRI-β subunit. Besides IgG, FcγRIIIA can be triggered also by oligomeric IgE. FcγRIIIB is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.
UsageThe reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working usage is 2·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J·Clin·Pathol. 1993·Apr; 46(4):334‑6. <·PMID:·7684403·> | Gessner JE, Grussenmeyer T, Kolanus W, Schmidt RE: The human low affinity immunoglobulin G Fc receptor III‑A and III‑B genes: Molecular characterization of the promoter regions. J·Biol·Chem. 1995·Jan·20; 270(3):1350‑61. <·PMID:·7836402·> | de Haas M, Koene HR, Kleijer M, de Vries E, Simsek S, van Tol MJ, Roos D, von dem Borne AE: A triallelic Fc gamma receptor type IIIA polymorphism influences the binding ofhuman IgG by NK cell Fc gamma RIIIa. J·Immunol. 1996·Apr·15; 156(8):2948‑55. <·PMID:·8609432·> | Tamm A, Schmidt RE: The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies: Implications for ligand binding. J·Immunol. 1996·Aug·15; 157(4):1576‑81. <·PMID:·8759741·> | Koene HR, Kleijer M, Algra J, Roos D, von dem Borne AE, de Haas M: Fc gammaRIIIa‑158V/F polymorphism influences the binding of IgG by natural killer cell Fc gammaRIIIa, independently of the Fc gammaRIIIa‑48L/R/H phenotype. Blood. 1997·Aug·1; 90(3):1109‑14. <·PMID:·9242542·> | Kocher M, Siegel ME, Edberg JC, Kimberly RP: Cross‑linking of Fc gamma receptor IIa and Fc gamma receptor IIIb induces different proadhesive phenotypes on human neutrophils. J·Immunol. 1997·Oct·15; 159(8):3940‑8. <·PMID:·9378982·> | Arase N, Arase H, Hirano S, Yokosuka T, Sakurai D, Saito T: IgE‑mediated activation of NK cells through Fc gamma RIII. J·Immunol. 2003·Mar·15; 170(6):3054‑8. <·PMID:·12626560·> | Gasdaska JR, Sherwood S, Regan JT, Dickey LF: An afucosylated anti‑CD20 monoclonal antibody with greater antibody‑dependent cellular cytotoxicity and B‑cell depletion and lower complement‑dependent cytotoxicity than rituximab. Mol·Immunol. 2012·Mar; 50(3):134‑41. <·PMID:·22305040·>

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