CyFlow™ CD16 APC

CyFlow™ CD16 APC
Laser: Red
Target Species: Human, Non-Human Primates
Antigen: CD16
Field of Interest: Immunophenotyping
Clone: 3G8
Regulatory Status: RUO
Alternative Name: CD16a, FcγRIIIA
Application: Flow cytometry
Format/Fluorochrome: APC
Species of Origin: Mouse
Clonality: monoclonal
Emission Maximum: 660 nm
Excitation Maximum: 650 nm
Isotype: IgG1
Product number: BT867914

For Research Use Only

$255.00 USD*

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HLDA Workshop HLDA V—WS Code NK80 Quantity 100 tests Immunogen Human neutrophils... more
CyFlow™ CD16 APC
HLDA WorkshopHLDA V—WS Code NK80
Quantity100 tests
ImmunogenHuman neutrophils
SpecifityThe mouse monoclonal antibody 3G8 recognizes CD16 antigen, a low affinity receptor for aggregated IgG (FcγRIII antigen). CD16 exists in two different isoforms: CD16a (FcγRIIIA; 50-65 kDa; expressed on NK-cells, monocytes and macrophages) and CD16b (FcγRIIIB; 48 kDa; mainly expressed on neutrophils).
Background InformationCD16 (FcγRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcγRIII is expressed in two forms - FcγRIIIA and FcγRIIIB. FcγRIIIA is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcεRI-γ subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcγRIIIA is associated, moreover, with FcεRI-β subunit. Besides IgG, FcγRIIIA can be triggered also by oligomeric IgE. FcγRIIIB is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.1% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

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