CyFlow™ CD14 APC

CyFlow™ CD14 APC
Laser: Red
Target Species: Human, Non-Human Primates
Antigen: CD14
Field of Interest: Immunophenotyping
Clone: MEM-18
Regulatory Status: RUO
Alternative Name: LPS-R
Application: Flow cytometry
Format/Fluorochrome: APC
Species of Origin: Mouse
Clonality: monoclonal
Emission Maximum: 660 nm
Excitation Maximum: 650 nm
Isotype: IgG1
Product number: BF508696

For Research Use Only

$255.00 USD*

Price excludes any applicable taxes plus shipping costs

Quantity 100 tests Immunogen A crude mixture of human urinary proteins precipitated by... more
CyFlow™ CD14 APC
Quantity100 tests
ImmunogenA crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria
SpecifityThe mouse monoclonal antibody MEM-18 recognizes CD14 antigen, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages. In human, the epitope recognized by MEM-18 is located between amino acids 57-64.
Background InformationCD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor; it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.1% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Bazil V, Horejsi V, Baudys M, Kristofova H, Strominger JL, Kostka W, Hilgert I: Biochemical characterization of a soluble form of the 53‑kDa monocyte surface antigen. Eur·J·Immunol. 1986·Dec; 16(12):1583‑9. <·PMID:·3493149·> | McMichael AJ, Beverley PCL, Cobbold S, et al (Eds): Leucocyte Typing III, White Cell Differentiation Antigens. Oxford·University·Press,·Oxford. 1987; 1‑1050. <·NLM·ID:·8913266·> | Knapp W, Dorken B, Gilks W, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (Eds): Leucocyte Typing IV. Oxford·University·Press,·Oxford. 1989; 1‑1820. <·NLM·ID:·8914679·> | Bazil V, Baudys M, Hilgert I, Stefanova I, Low MG, Zbrozek J, Horejsi V: Structural relationship between the soluble and membrane‑bound forms of human monocyte surface glycoprotein CD14. Mol·Immunol. 1989·Jul; 26(7):657‑62. <·PMID:·2779588·> | Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J·Clin·Pathol. 1993·Apr; 46(4):334‑6. <·PMID:·7684403·> | Juan TS, Hailman E, Kelley MJ, Wright SD, Lichenstein HS: Identification of a domain in soluble CD14 essential for lipopolysaccharide (LPS) signaling but not LPS binding. J·Biol·Chem. 1995·Jul·21; 270(29):17237‑42. <·PMID:·7542233·> | Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland·Publishing·Inc,·New·York. 1997; 1‑1342. <·NLM·ID:·9712219·> | Funda DP, Tucková L, Farré MA, Iwase T, Moro I, Tlaskalová-Hogenová H: CD14 is expressed and released as soluble CD14 by human intestinal epithelial cells in vitro: lipopolysaccharide activation of epithelial cells revisited. Infect·Immun. 2001·Jun; 69(6):3772‑81. <·PMID:·11349042·> | Stöckl J, Majdic O, Fischer G, Maurer D, Knapp W: Monomorphic molecules function as additional recognition structures on haptenated target cells for HLA‑A1‑restricted, hapten‑specific CTL. J·Immunol. 2001·Sep·1; 167(5):2724‑33. <·PMID:·11509616·> | Sing A, Rost D, Tvardovskaia N, Roggenkamp A, Wiedemann A, Kirschning CJ, Aepfelbacher M, Heesemann J: Yersinia V‑antigen exploits toll‑like receptor 2 and CD14 for interleukin 10‑mediated immunosuppression. J·Exp·Med. 2002·Oct·21; 196(8):1017‑24. <·PMID:·12391013·> | Fernández-Real JM, Broch M, Richart C, Vendrell J, López-Bermejo A, Ricart W: CD14 monocyte receptor, involved in the inflammatory cascade, and insulin sensitivity. J·Clin·Endocrinol·Metab. 2003·Apr; 88(4):1780‑4. <·PMID:·12679473·> | Iwaki D, Nishitani C, Mitsuzawa H, Hyakushima N, Sano H, Kuroki Y: The CD14 region spanning amino acids 57‑64 is critical for interaction with the extracellular Toll‑like receptor 2 domain. Biochem·Biophys·Res·Commun. 2005·Mar·4; 328(1):173‑6. <·PMID:·15670766·> | Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single‑molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int·Immunol. 2007·May; 19(5):675‑84. <·PMID:·17446208·> | Angel CE, Lala A, Chen CJ, Edgar SG, Ostrovsky LL, Dunbar PR: CD14+ antigen‑presenting cells in human dermis are less mature than their CD1a+ counterparts. Int·Immunol. 2007·Nov; 19(11):1271‑9. <·PMID:·17804688·> | Lodrup Carlsen KC, Granum B: Soluble CD14: role in atopic disease and recurrent infections, including otitis media. Curr·Allergy·Asthma·Rep. 2007·Nov; 7(6):436‑43. <·PMID:·17986374·> | Asai Y, Makimura Y, Kawabata A, Ogawa T: Soluble CD14 discriminates slight structural differences between lipid as that lead to distinct host cell activation. J·Immunol. 2007·Dec·1; 179(11):7674‑83. <·PMID:·18025213·> | Weiss TS, Lichtenauer M, Kirchner S, Stock P, Aurich H, Christ B, Brockhoff G, Kunz-Schughart LA, Jauch KW, Schlitt HJ, Thasler WE: Hepatic progenitor cells from adult human livers for cell transplantation. Gut. 2008·Aug; 57(8):1129‑38. <·PMID:·18417531·>

Article successfully added.