CyFlow™ CD11b Low Endotoxin

CyFlow™ CD11b Low Endotoxin
Target Species: Human, Mouse, Non-Human Primates, Rabbit
Antigen: CD11b
Field of Interest: Immunophenotyping, MHC
Clone: M1/70
Regulatory Status: RUO
Alternative Name: Mac-1
Application: Flow cytometry, Functional assays, Immunohistochemistry (frozen sections), Immunoprecipitation
Format/Fluorochrome: Low Endotoxin
Species of Origin: Rat
Clonality: monoclonal
Isotype: IgG2b
Product number: AX566986

For Research Use Only

$130.00 USD*

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Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Immunogen B10 mouse... more
CyFlow™ CD11b Low Endotoxin
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
ImmunogenB10 mouse spleen cells enriched for T cells
SpecifityThe rat monoclonal antibody M1/70 recognizes CD11b antigen, a type I transmembrane protein mainly expressed on monocytes/macrophages, granulocytes and NK-cells, which associates with CD18 to form Mac-1 integrin that plays important role in cell-cell interactions.
Background InformationCD11b (integrin αM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with CD18 (integrin β2 subunit); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
Storage BufferThe reagent is provided in azide-free phosphate buffered saline (PBS) solution, pH ≈7.4; 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

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