CyFlow™ CD11b APC

CyFlow™ CD11b APC
Laser: Red
Target Species: Human, Mouse, Non-Human Primates, Rabbit
Antigen: CD11b
Field of Interest: Immunophenotyping, MHC
Clone: M1/70
Regulatory Status: RUO
Alternative Name: Mac-1
Application: Flow cytometry
Format/Fluorochrome: APC
Species of Origin: Rat
Clonality: monoclonal
Emission Maximum: 660 nm
Excitation Maximum: 650 nm
Isotype: IgG2b
Product number: BR751578

For Research Use Only

$255.00 USD*

Price excludes any applicable taxes plus shipping costs

Concentration 0.5 mg/mL Quantity 0.1 mg Volume 0.2 mL Immunogen B10 mouse spleen... more
CyFlow™ CD11b APC
Concentration0.5 mg/mL
Quantity0.1 mg
Volume0.2 mL
ImmunogenB10 mouse spleen cells enriched for T cells
Background InformationCD11b (integrin αM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with CD18 (integrin β2 subunit); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
UsageThe reagent is designed for Flow Cytometry analysis. Working concentrations should be determined by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Springer T, Galfrè G, Secher DS, Milstein C: Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur·J·Immunol. 1978·Aug; 8(8):539‑51. <·PMID:·81133·> | Ault KA, Springer TA: Cross‑reaction of a rat‑anti‑mouse phagocyte‑specific monoclonal antibody (anti‑Mac‑{01} with human monocytes and natural killer cells. J·Immunol. 1981·Jan; 126(1):359‑64. <·PMID:·7451976·> | Sanchez-Madrid F, Simon P, Thompson S, Springer TA: Mapping of antigenic and functional epitopes on the alpha‑ and beta‑subunits of two related mouse glycoproteins involved in cell interactions, LFA‑1 and Mac‑1. J·Exp·Med. 1983·Aug·1; 158(2):586‑602. <·PMID:·6193226·> | Whiteland JL, Nicholls SM, Shimeld C, Easty DL, Williams NA, Hill TJ: Immunohistochemical detection of T‑cell subsets and other leukocytes in paraffin‑embedded rat and mouse tissues with monoclonal antibodies. J·Histochem·Cytochem. 1995·Mar; 43(3):313‑20. <·PMID:·7868861·> | Dembic Z, Schenck K, Bogen B: Dendritic cells purified from myeloma are primed with tumor‑specific antigen (idiotype) and activate CD4+ T cells. Proc·Natl·Acad·Sci·USA. 2000·Mar·14; 97(6):2697‑702. <·PMID:·10706628·> | Welt FG, Edelman ER, Simon DI, Rogers C: Neutrophil, not macrophage, infiltration precedes neointimal thickening in balloon‑injured arteries. Arterioscler·Thromb·Vasc·Biol. 2000·Dec; 20(12):2553‑8. <·PMID:·11116052·> | Zhang Y, McCormick LL, Desai SR, Wu C, Gilliam AC: Murine sclerodermatous graft‑versus‑host disease, a model for human scleroderma: cutaneous cytokines, chemokines, and immune cell activation. J·Immunol. 2002·Mar·15; 168(6):3088‑98. <·PMID:·11884483·> | Brickson S, Ji LL, Schell K, Olabisi R, St Pierre Schneider B, Best TM: M1/70 attenuates blood‑borne neutrophil oxidants, activation, and myofiber damage following stretch injury. J·Appl·Physiol. 2003·Sep; 95(3):969‑76. <·PMID:·12730143·> | Leon F, Contractor N, Fuss I, Marth T, Lahey E, Iwaki S, la Sala A, Hoffmann V, Strober W, Kelsall BL: Antibodies to complement receptor 3 treat established inflammation in murine models of colitis and a novel model of psoriasiform dermatitis. J·Immunol. 2006·Nov·15; 177(10):6974‑82. <·PMID:·17082612·> | Takagi H, Numazaki M, Kajiwara T, Abe Y, Ishii M, Kato C, Kojima N: Cooperation of specific ICAM‑3 grabbing nonintegrin‑related 1 (SIGNR{01} and complement receptor type 3 (CR3) in the uptake of oligomannose‑coated liposomes by macrophages. Glycobiology. 2009·Mar; 19(3):258‑66. <·PMID:·19029201·>

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