CyFlow™ CD11a Azide Free

CyFlow™ CD11a Azide Free
Target Species: Human
Antigen: CD11a
Field of Interest: Immunophenotyping
Clone: MEM-25
Regulatory Status: RUO
Alternative Name: LFA-1
Application: Flow cytometry, Functional assays, Immunoprecipitation
Format/Fluorochrome: Azide Free
Species of Origin: Mouse
Clonality: monoclonal
Isotype: IgG1
Product number: AM553938

For Research Use Only

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Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Immunogen Leukocytes of... more
CyFlow™ CD11a Azide Free
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
ImmunogenLeukocytes of a patient suffering from LGL-type Leukemia
SpecifityThe mouse monoclonal antibody MEM-25 recognizes CD11a antigen, a 170-180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.
Background InformationCD11a (LFA-1 α) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the αLβ2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation; binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.
Storage BufferThe reagent is provided in azide-free phosphate buffered saline (PBS) solution, pH ≈7.4; 0.2 µm filter sterilized.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

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