CyFlow™ CD11a FITC

CyFlow™ CD11a FITC
Laser: Blue
Target Species: Mouse
Antigen: CD11a
Field of Interest: Immunophenotyping, MHC
Clone: M17/4
Regulatory Status: RUO
Alternative Name: LFA-1
Application: Flow cytometry
Format/Fluorochrome: FITC
Species of Origin: Rat
Clonality: monoclonal
Emission Maximum: 518 nm
Excitation Maximum: 490 to 495 nm
Isotype: IgG2a
Product number: CK020205

For Research Use Only

$235.00 USD*

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Concentration 0.5 mg/mL Quantity 0.1 mg Volume 0.2 mL Immunogen C57BL/6 mouse... more
CyFlow™ CD11a FITC
Concentration0.5 mg/mL
Quantity0.1 mg
Volume0.2 mL
ImmunogenC57BL/6 mouse splenic secondary cytotoxic T lymphocytes
Background InformationCD11a (LFA-1 α) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the αLβ2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions.
UsageThe reagent is designed for Flow Cytometry analysis. Suggested working usage is 3·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Sanchez-Madrid F, Davignon D, Martz E, Springer TA: Antigens involved in mouse cytolytic T‑lymphocyte (CTL)‑mediated killing: functional screening and topographic relationship. Cell·Immunol. 1982·Oct; 73(1):1-11. <·PMID:·6983917·> | Kuhlman P, Moy VT, Lollo BA, Brian AA: The accessory function of murine intercellular adhesion molecule‑1 in T lymphocyte activation: Contributions of adhesion and co‑activation. J·Immunol. 1991·Mar·15; 146(6):1773‑82. <·PMID:·1672331·> | Yung R, Powers D, Johnson K, Amento E, Carr D, Laing T, Yang J, Chang S, Hemati N, Richardson B: Mechanisms of drug‑induced lupus (II) T cells overexpressing lymphocyte function‑associated antigen 1 become autoreactive and cause a lupus‑like disease in syngeneic mice. J·Clin·Invest. 1996·Jun·15; 97(12):2866‑71. <·PMID:·8675699·> | Papayannopoulou T, Priestley GV, Nakamoto B, Zafiropoulos V, Scott LM, Harlan JM: Synergistic mobilization of hemopoietic progenitor cells using concurrent beta1 and beta2 integrin blockade or beta2‑deficient mice. Blood. 2001·Mar·1; 97(5):1282‑8. <·PMID:·11222371·> | Koseki S, Miura S, Fujimori H, Hokari R, Komoto S, Hara Y, Ogino T, Nagata H, Goto M, Hachimura S, Kaminogawa S, Ishii H: In situ demonstration of intraepithelial lymphocyte adhesion to villus microvessels of the small intestine. Int·Immunol. 2001·Sep; 13(9):1165‑74. <·PMID:·11526097·> | Bellingan GJ, Xu P, Cooksley H, Cauldwell H, Shock A, Bottoms S, Haslett C, Mutsaers SE, Laurent GJ: Adhesion molecule‑dependent mechanisms regulate the rate of macrophage clearance during the resolution of peritoneal inflammation. J·Exp·Med. 2002·Dec·2; 196(11):1515‑21. <·PMID:·12461086·> | Beyer M, Wang H, Peters N, Doths S, Koerner-Rettberg C, Openshaw PJ, Schwarze J: The beta2 integrin CD11c distinguishes a subset of cytotoxic pulmonary T cells with potent antiviral effects in vitro and in vivo. Respir·Res. 2005·Jul·12; 6:70. <·PMID:·16011799·> | Angelov GS, Guillaume P, Cebecauer M, Bosshard G, Dojcinovic D, Baumgaertner P, Luescher IF: Soluble MHC‑peptide complexes containing long rigid linkers abolish CTL‑mediated cytotoxicity. J·Immunol. 2006·Mar·15; 176(6):3356‑65. <·PMID:·16517703·> | Smith SS, Barnum SR: Differential expression of beta 2‑integrins and cytokine production between gammadelta and alphabeta T cells in experimental autoimmune encephalomyelitis. J·Leukoc·Biol. 2008·Jan; 83(1):71‑9. <·PMID:·17928460·>

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