CyFlow™ CD10 Purified

CyFlow™ CD10 Purified
Target Species: Human
Antigen: CD10
Field of Interest: Immunophenotyping
Clone: MEM-78
Regulatory Status: RUO
Alternative Name: CALLA, gp100, NEP
Application: Flow cytometry, Immunoprecipitation
Format/Fluorochrome: Purified
Species of Origin: Mouse
Clonality: monoclonal
Isotype: IgG1
Product number: BT538992

For Research Use Only

$120.00 USD*

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HLDA Workshop HLDA IV—WS Code B 506 HLDA V—WS Code B CD10.4 Concentration Unit mg/mL... more
CyFlow™ CD10 Purified
HLDA WorkshopHLDA IV—WS Code B 506 HLDA V—WS Code B CD10.4
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
ImmunogenNALM-6 human pre-B cell line
SpecifityThe mouse monoclonal antibody MEM-78 recognizes CD10 antigen, a 100 kDa type II integral membrane protein.
Background InformationCD10 (neutral endopeptidase - NEP, common acute lymphocytic leukemia antigen - CALLA, membrane metallo-endopeptidase - MME, enkefalinase) is a 100 kDa cell surface zinc metalloprotease cleaving peptide bonds on the N-terminus of hydrophobic amino acids and inactivating multiple physiologically active peptides. CD10 is expressed on various normal cell types, including lymphoid precursor cells, germinal center B lymhocytes, and some epithelial cells, and its expression level serves as a marker for diagnostics of many carcinomas. CD10 is also a differentiation antigen for early B-lymphoid progenitors in the B-cell differentiation pathway and has a key role in regulation of growth, differentiation and signal transduction of many cellular systems.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.1% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Horejsí V, Angelisová P, Bazil V, Kristofová H, Stoyanov S, Stefanová I, Hausner P, Vosecký M, Hilgert I: Monoclonal antibodies against human leucocyte antigens (II); Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18‑kDa antigen (MEM‑43) and a novel antigen of restricted expression (MEM‑74). Folia·Biol·(Praha). 1988; 34(1):23‑34. <·PMID:·2968928·> | Knapp W, Dorken B, Gilks W, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (Eds): Leucocyte Typing IV. Oxford·University·Press,·Oxford. 1989; 1‑1820. <·NLM·ID:·8914679·> | Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J·Clin·Pathol. 1993·Apr; 46(4):334‑6. <·PMID:·7684403·> | Angelisová P, Drbal K, Cerny J, Hilgert I, Horejsi V: Characterization of the human leukocyte GPI‑anchored glycoprotein CDw108 and its relation to other similar molecules. Immunobiology. 1999·Jun; 200(2):234‑45. <·PMID:·10416131·> | Suzuki T, Ino K, Kikkawa F, Uehara C, Kajiyama H, Shibata K, Mizutani S: Neutral endopeptidase/CD10 expression during phorbol ester‑induced differentiation of choriocarcinoma cells through the protein kinase C‑ and extracellular signal‑regulated kinase‑dependent signalling pathway. Placenta. 2002·Jul; 23(6):475‑82. <·PMID:·12137745·> | Yada K, Kashima K, Daa T, Kitano S, Fujiwara S, Yokoyama S: Expression of CD10 in basal cell carcinoma. Am·J·Dermatopathol. 2004·Dec; 26(6):463‑71. <·PMID:·15618927·> | Braham H, Trimeche M, Ziadi S, Mestiri S, Mokni M, Amara K, Hachana M, Sriha B, Korbi S: CD10 expression by fusiform stromal cells in nasopharyngeal carcinoma correlates with tumor progression. Virchows·Arch. 2006·Aug; 449(2):220‑4. <·PMID:·16673118·> | Dall'Era MA, True LD, Siegel AF, Porter MP, Sherertz TM, Liu AY: Differential expression of CD10 in prostate cancer and its clinical implication. BMC·Urol. 2007·Mar·2; 7:3. <·PMID:·17335564·>

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