CyFlow™ CD8a APC

CyFlow™ CD8a APC
Laser: Red
Target Species: Mouse
Antigen: CD8a
Field of Interest: Immunophenotyping, MHC
Clone: 53-6.7
Regulatory Status: RUO
Alternative Name: Leu2, T8
Application: Flow cytometry
Format/Fluorochrome: APC
Species of Origin: Rat
Clonality: monoclonal
Emission Maximum: 660 nm
Excitation Maximum: 650 nm
Isotype: IgG2a
Product number: AH990077

For Research Use Only

$255.00 USD*

Price excludes any applicable taxes plus shipping costs

Concentration 0.5 mg/mL Quantity 0.1 mg Volume 0.2 mL Immunogen Mouse spleen... more
CyFlow™ CD8a APC
Concentration0.5 mg/mL
Quantity0.1 mg
Volume0.2 mL
ImmunogenMouse spleen cells
Background InformationCD8a (CD8 α) subunit of CD8 T cell coreceptor is expressed in CD8 α/β heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 α/α homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages and dendritic cells. Regulation of CD8 β level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 α/β but not α/α dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
UsageThe reagent is designed for Flow Cytometry analysis. Suggested working usage is 3·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Ledbetter JA, Herzenberg LA: Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol·Rev. 1979; 47:63‑90. <·PMID:·398327·> | Ledbetter JA, Rouse RV, Micklem HS, Herzenberg LA: T cell subsets defined by expression of Lyt‑1,2,3 and Thy‑1 antigens: Two‑parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. J·Exp·Med. 1980·Aug·1; 152(2):280‑95. <·PMID:·6156984·> | Takahashi K, Nakata M, Tanaka T, Adachi H, Nakauchi H, Yagita H, Okumura K: CD4 and CD8 regulate interleukin 2 responses of T cells. Proc·Natl·Acad·Sci·USA. 1992·Jun·15; 89(12):5557‑61. <·PMID:·1608966·> | Grabbe S, Varga G, Beissert S, Steinert M, Pendl G, Seeliger S, Bloch W, Peters T, Schwarz T, Sunderkötter C, Scharffetter-Kochanek K: Beta2 integrins are required for skin homing of primed T cells but not for priming naive T cells. J·Clin·Invest. 2002·Jan; 109(2):183‑92. <·PMID:·11805130·> | Hata H, Sakaguchi N, Yoshitomi H, Iwakura Y, Sekikawa K, Azuma Y, Kanai C, Moriizumi E, Nomura T, Nakamura T, Sakaguchi S: Distinct contribution of IL‑6, TNF‑alpha, IL‑1, and IL‑10 to T cell‑mediated spontaneous autoimmune arthritis in mice. J·Clin·Invest. 2004·Aug; 114(4):582‑8. <·PMID:·15314695·> | Ko SY, Ko HJ, Chang WS, Park SH, Kweon MN, Kang CY: alpha‑Galactosylceramide can act as a nasal vaccine adjuvant inducing protective immune responses against viral infection and tumor. J·Immunol. 2005·Sep·1; 175(5):3309‑17. <·PMID:·16116223·> | Bouwer HG, Alberti-Segui C, Montfort MJ, Berkowitz ND, Higgins DE: Directed antigen delivery as a vaccine strategy for an intracellular bacterialpathogen. Proc·Natl·Acad·Sci·USA. 2006·Mar·28; 103(13):5102‑7. <·PMID:·16549792·> | Kamimura D, Sawa Y, Sato M, Agung E, Hirano T, Murakami M: IL‑2 in vivo activities and antitumor efficacy enhanced by an anti‑IL‑2 mAb. J·Immunol. 2006·Jul·1; 177(1):306‑14. <·PMID:·16785526·> | Mochimaru H, Usui T, Yaguchi T, Nagahama Y, Hasegawa G, Usui Y, Shimmura S, Tsubota K, Amano S, Kawakami Y, Ishida S: Suppression of alkali burn‑induced corneal neovascularization by dendritic cell vaccination targeting VEGF receptor 2. Invest·Ophthalmol·Vis·Sci. 2008·May; 49(5):2172‑7. <·PMID:·18263815·>

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