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CyFlow™ CD8 Biotin

CyFlow™ CD8 Biotin
Antibody: Yes
Antigen: CD8
Application: Flow cytometry
Clonality: monoclonal
Clone: MEM-31
Field of Interest: Immunophenotyping
Format/Fluorochrome: Biotin
Isotype: IgG2a
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: AM060913

For Research Use Only

$160.00 USD*

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HLDA Workshop HLDA III—WS Code T 575 Concentration Unit mg/mL Concentration 1... more
CyFlow™ CD8 Biotin
HLDA WorkshopHLDA III—WS Code T 575
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenCrude thymus membrane fraction
Background InformationThe CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as α/β heterodimer on majority of MHC I-restricted conventional T cells and thymocytes and as α/α homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells. Regulation of CD8 β level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 α-β but not α-α dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
UsageThe reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working usage is 0.4·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

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