CyFlow™ CD4 PerCP

CyFlow™ CD4 PerCP
Laser: Blue, Green
Target Species: Human
Antigen: CD4
Field of Interest: Immunophenotyping
Clone: MEM-241
Regulatory Status: RUO
Alternative Name: Leu3a, T4
Application: Flow cytometry
Format/Fluorochrome: PerCP
Species of Origin: Mouse
Clonality: monoclonal
Emission Maximum: 678 nm
Excitation Maximum: 482 to 490 nm
Isotype: IgG1
Product number: AS415231

For Research Use Only

$265.00 USD*

Price excludes any applicable taxes plus shipping costs

HLDA Workshop HCDM (former HLDA VIII)—WS Code M241 Quantity 100 tests Immunogen 2... more
CyFlow™ CD4 PerCP
HLDA WorkshopHCDM (former HLDA VIII)—WS Code M241
Quantity100 tests
Immunogen2 N-terminal domains of human CD4 fused to human IgG1 Fc
SpecifityThe mouse monoclonal antibody MEM-241 recognizes CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes (''helper'' T-cells) and also on monocytes, tissue macrophages and granulocytes.
Background InformationCD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin . Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.1% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Millan J, Cerny J, Horejsi V, Alonso MA: CD4 segregates into specific detergent‑resistant T‑cell membrane microdomains. Tissue·Antigens. 1999·Jan; 53(1):33‑40. <·PMID:·10082429·> | Foti M, Phelouzat MA, Holm A, Rasmusson BJ, Carpentier JL: p56Lck anchors CD4 to distinct microdomains on microvilli. Proc·Natl·Acad·Sci·USA. 2002·Feb·19; 99(4):2008‑13. <·PMID:·11854499·> | Clapham PR, McKnight A: Cell surface receptors, virus entry and tropism of primate lentiviruses. . J·Gen·Virol. 2002·Aug; 83(8):1809‑29. <·PMID:·12124446·> | Brdicková N, Brdicka T, Angelisová P, Horváth O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S, Merten C, Schraven B, Horejsí V: LIME: a new membrane Raft‑associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J·Exp·Med. 2003·Nov·17; 198(10):1453‑62. <·PMID:·14610046·> | Zola H, Swart B, Banham A, Barry S, Beare A, Bensussan A, Boumsell L, D Buckley C, Buhring HJ, Clark G, Engel P, Fox D, Jin BQ, Macardle PJ, Malavasi F, Mason D, Stockinger H, Yang X: CD molecules 2006: human cell differentiation molecules. J·Immunol·Methods. 2007·Jan·30; 319(1‑2):1‑5. <·PMID:·17174972·> | Manasa J, Musabaike H, Masimirembwa C, Burke E, Luthy R, Mudzori J: Evaluation of the Partec flow cytometer against the BD FACSCalibur system for monitoring immune responses of human immunodeficiency virus‑infected patients in Zimbabwe. Clin·Vaccine·Immunol. 2007·Mar; 14(3):293‑8. <·PMID:·17267593·> | Anderson AE, Sayers BL, Haniffa MA, Swan DJ, Diboll J, Wang XN, Isaacs JD, Hilkens CM: Differential regulation of naïve and memory CD4+ T cells by alternatively activated dendritic cells. J·Leukoc·Biol. 2008·Jul; 84(1):124‑33. <·PMID:·18430785·> | Karlsson KR, Cowley S, Martinez FO, Shaw M, Minger SL, James W: Homogeneous monocytes and macrophages from human embryonic stem cells following coculture‑free differentiation in M‑CSF and IL‑3. Exp·Hematol. 2008·Sep; 36(9):1167‑75. <·PMID:·18550257·> | Hovden AO, Karlsen M, Jonsson R, Aarstad HJ, Appel S: Maturation of monocyte derived dendritic cells with OK432 boosts IL‑12p70 secretion and conveys strong T‑cell responses. BMC·Immunol. 2011·Jan·5; 12:2. <·PMID:·21208424·> | Kanderova V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, Hrusak O, Lund‐Johansen F, Kalina T: High‐resolution antibody array analysis of childhood acute leukemia cells. Mol·Cell·Proteomics. 2016·Apr·1; 15(4):1246‐61. <·PMID:·26785729·>

Article successfully added.