CyFlow™ CD4 FITC

CyFlow™ CD4 FITC
Laser: Blue
Target Species: Mouse
Antigen: CD4
Field of Interest: Immunophenotyping, MHC
Clone: GK1.5
Regulatory Status: RUO
Alternative Name: Leu3a, T4
Application: Flow cytometry
Format/Fluorochrome: FITC
Species of Origin: Rat
Clonality: monoclonal
Emission Maximum: 518 nm
Excitation Maximum: 490 to 495 nm
Isotype: IgG2b
Product number: AZ163960

For Research Use Only

$235.00 USD*

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HLDA Workshop HLDA V—WS Code T T-CD04.10 HLDA VI—WS Code T 6T-013 Concentration Unit... more
CyFlow™ CD4 FITC
HLDA WorkshopHLDA V—WS Code T T-CD04.10 HLDA VI—WS Code T 6T-013
Concentration Unitmg/mL
Concentration0.5
Quantity0.1 mg
ImmunogenMouse CTL clone V4 cells
SpecifityThe rat monoclonal antibody GK1.5 recognizes an extracellular epitope of mouse CD4 transmembrane glycoprotein (55 kDa).
Background InformationCD4 is a single chain transmembrane glycoprotein of immunoglobulin supergene family. In its extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). The intracellular region of CD4 associates with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. CD4 binds to MHC class II molecules (by CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (by CDR2-like region in CD4 domain 1) and other ligands, such as IL-16 (by to CD4 domain 3) or L-selectin. CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection. CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
UsageThe reagent is designed for Flow Cytometry analysis. Suggested working usage is 3·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.1% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Dialynas DP, Wilde DB, Marrack P, Pierres A, Wall KA, Havran W, Otten G, Loken MR, Pierres M, Kappler J, et al: Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with class II MHC antigen‑reactivity. Immunol·Rev. 1983; 74:29‑56. <·PMID:·6195085·> | Dialynas DP, Quan ZS, Wall KA, Pierres A, Quintáns J, Loken MR, Pierres M, Fitch FW: Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu‑3/T4 molecule. J·Immunol. 1983·Nov; 131(5):2445‑51. <·PMID:·6415170·> | Wilde DB, Marrack P, Kappler J, Dialynas DP, Fitch FW: Evidence implicating L3T4 in class II MHC antigen reactivity: monoclonal antibody GK1.5 (anti‑L3T4a) blocks class II MHC antigen‑specific proliferation, release of lymphokines, and binding by cloned murine helper T lymphocyte lines. J·Immunol. 1983·Nov; 131(5):2178‑83. <·PMID:·6195255·> | Wu L, Antica M, Johnson GR, Scollay R, Shortman K: Developmental potential of the earliest precursor cells from the adult mouse thymus. J·Exp·Med. 1991·Dec·1; 174(6):1617‑27. <·PMID:·1683894·> | Godfrey DI, Kennedy J, Gately MK, Hakimi J, Hubbard BR, Zlotnik A: IL‑12 influences intrathymic T cell development. J·Immunol. 1994·Mar·15; 152(6):2729‑35. <·PMID:·7511624·> | Gavett SH, Chen X, Finkelman F, Wills-Karp M: Depletion of murine CD4+ T lymphocytes prevents antigen‑induced airway hyperreactivity and pulmonary eosinophilia. Am·J·Respir·Cell·Mol·Biol. 1994·Jun; 10(6):587‑93. <·PMID:·8003337·> | Zheng B, Han S, Kelsoe G: T helper cells in murine germinal centers are antigen‑specific emigrants that downregulate Thy‑1. J·Exp·Med. 1996·Sep·1; 184(3):1083‑91. <·PMID:·9064325·> | Felix NJ, Donermeyer DL, Horvath S, Walters JJ, Gross ML, Suri A, Allen PM: Alloreactive T cells respond specifically to multiple distinct peptide‑MHC complexes. Nat·Immunol. 2007·Apr; 8(4):388‑97. <·PMID:·17322886·> | Yi H, Zhen Y, Zeng C, Zhang L, Zhao Y: Depleting anti‑CD4 monoclonal antibody (GK1.5) treatment: influence on regulatory CD4+CD25+Foxp3+ T cells in mice. Transplantation. 2008·Apr·27; 85(8):1167‑74. <·PMID:·18431238·> | Hu M, Watson D, Zhang GY, Graf N, Wang YM, Sartor M, Howden B, Fletcher J, Alexander SI: Long‑term cardiac allograft survival across an MHC mismatch after "pruning" of alloreactive CD4 T cells. J·Immunol. 2008·May·15; 180(10):6593‑603. <·PMID:·18453578·>

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