CyFlow™ CD3 Low Endotoxin

CyFlow™ CD3 Low Endotoxin
Target Species: Human
Antigen: CD3
Field of Interest: Immunophenotyping
Clone: MEM-92
Regulatory Status: RUO
Alternative Name: Leu4, T3
Application: Flow cytometry, Functional assays, Immunoprecipitation
Format/Fluorochrome: Low Endotoxin
Species of Origin: Mouse
Clonality: monoclonal
Isotype: IgM
Product number: BB372557

For Research Use Only

$130.00 USD*

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HLDA Workshop HLDA IV—WS Code T 97 Concentration Unit mg/mL Concentration 1... more
CyFlow™ CD3 Low Endotoxin
HLDA WorkshopHLDA IV—WS Code T 97
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
ImmunogenHuman thymocytes and T lymphocytes
SpecifityThe mouse monoclonal antibody MEM-92 recognizes ε chain of human CD3 complex, a part of a bigger multisubunit complex of the T cell receptor (CD3/TCR) expressed on peripheral blood T lymphocytes and mature thymocytes.
Background InformationCD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 γ, CD3 δ, CD3 ε and CD3 ζ. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Storage BufferThe reagent is provided in azide-free phosphate buffered saline (PBS) solution, pH ≈7.4; 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

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