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CyFlow™ CD3 Low Endotoxin

CyFlow™ CD3 Low Endotoxin
Alternative Name: Leu4, T3
Antibody: Yes
Antigen: CD3
Application: Flow cytometry, Functional assays, Immunoprecipitation
Clonality: monoclonal
Clone: MEM-92
Field of Interest: Immunophenotyping
Format/Fluorochrome: Low Endotoxin
Isotype: IgM
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: BB372557

For Research Use Only

$130.00 USD*

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HLDA Workshop HLDA IV—WS Code T 97 Concentration Unit mg/mL Concentration 1... more
CyFlow™ CD3 Low Endotoxin
HLDA WorkshopHLDA IV—WS Code T 97
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenHuman thymocytes and T lymphocytes
Background InformationCD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3γ, CD3 δ, CD3ε and CD3ζ. These CD3 subunits are structurally related members of the immunoglobulins superfamily encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Storage BufferThe reagent is provided in azide-free phosphate buffered saline (PBS) solution, pH ≈7.4; 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Knapp W, Dorken B, Gilks W, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (Eds): Leucocyte Typing IV. Oxford University Press, Oxford. 1989; 1‑1820. < NLM ID: 8914679 > | Soucek J, Hilgert I, Budová I, Lindnerová G: Augmentation of NK cell activity and proliferation in cultured lymphocytes of leukemic patients by monoclonal antibodies CD3 and interleukin‑2. Neoplasma. 1994; 41(2):75‑81. < PMID: 8208318 > | Stefanová I, Saville MW, Peters C, Cleghorn FR, Schwartz D, Venzon DJ, Weinhold KJ, Jack N, Bartholomew C, Blattner WA, Yarchoan R, Bolen JB, Horak ID: HIV infection‑‑induced posttranslational modification of T cell signaling molecules associated with disease progression. J Clin Invest. 1996 Sep 15; 98(6):1290‑7. < PMID: 8823293 > | Brdicka T, Imrich M, Angelisova P, Brdickova N, Horvath O, Spicka J, Hilgert I, Luskova P, Draber P, Novak P, Engels N, Wienands J, Simeoni L, Osterreicher J, Aguado E, Malissen M, Schraven B, Horejsi V: Non‑T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling. J Exp Med. 2002 Dec 16; 196(12):1617‑26. < PMID: 12486104 > | Brdicková N, Brdicka T, Angelisová P, Horváth O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S, Merten C, Schraven B, Horejsí V: LIME: a new membrane Raft‑associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J Exp Med. 2003 Nov 17; 198(10):1453‑62. < PMID: 14610046 > | Stroncek DF, Caruccio L, Bettinotti M: CD177: A member of the Ly‑6 gene superfamily involved with neutrophil proliferation and polycythemia vera. J Transl Med. 2004 Mar 29; 2(1):8. < PMID: 15050027 > | Batista A, Millán J, Mittelbrunn M, Sánchez-Madrid F, Alonso MA: Recruitment of transferrin receptor to immunological synapse in response to TCR engagement. J Immunol. 2004 Jun 1; 172(11):6709‑14. < PMID: 15153487 > | Huang Y, Wange RL: T cell receptor signaling: beyond complex complexes. J Biol Chem. 2004 Jul 9; 279(28):28827‑3. < PMID: 15084594 > | Kuhns MS, Davis MM, Garcia KC: Deconstructing the form and function of the TCR/CD3 complex. Immunity. 2006 Feb; 24(2):133‑9. < PMID: 16473826 > | Alarcón B, Swamy M, van Santen HM, Schamel WW: T‑cell antigen‑receptor stoichiometry: pre‑clustering for sensitivity. EMBO Rep. 2006 May; 7(5):490‑5. < PMID: 16670682 > | Meraner P, Horejsí V, Wolpl A, Fischer GF, Stingl G, Maurer D: Dendritic cells sensitize TCRs through self‑MHC‑mediated Src family kinase activation. J Immunol. 2007 Feb 15; 178(4):2262‑71. < PMID: 17277131 >