CyFlow™ CD2 APC

CyFlow™ CD2 APC
Laser: Red
Target Species: Human
Antigen: CD2
Field of Interest: Immunophenotyping
Clone: LT2
Regulatory Status: RUO
Alternative Name: LFA-2
Application: Flow cytometry
Format/Fluorochrome: APC
Species of Origin: Mouse
Clonality: monoclonal
Emission Maximum: 660 nm
Excitation Maximum: 650 nm
Isotype: IgG2b
Product number: CG364305

For Research Use Only

$255.00 USD*

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HLDA Workshop HLDA VI—WS Code T 6T-008 Concentration Quantity 100 tests Volume... more
CyFlow™ CD2 APC
HLDA WorkshopHLDA VI—WS Code T 6T-008
Concentration
Quantity100 tests
Volume1.0 mL
ImmunogenNormal human blood lymphocytes
Background InformationCD2 (LFA-2) belongs to T lymphocyte glycoproteins of immunoglobulin superfamily. Its interaction with CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Filatov AV, Bachurin PS, Markova NA, Surnakova NE: A panel of monoclonal antibodies against human lymphocyte antigens. Eksp·Onkol. 1989; 11(2):28‑32. <·PMID:·2737128·> | Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland·Publishing·Inc,·New·York. 1997; 1‑1342. <·NLM·ID:·9712219·> | Dustin ML: Adhesive bond dynamics in contacts between T lymphocytes and glass‑supported planar bilayers reconstituted with the immunoglobulin‑related adhesion molecule CD58. J·Biol·Chem. 1997·Jun·20; 272(25):15782‑8. <·PMID:·9188475·> | Gannon GA, Rhind SG, Suzui M, Zamecnik J, Sabiston BH, Shek PN, Shephard RJ: beta‑Endorphin and natural killer cell cytolytic activity during prolonged exercise: Is there a connection?. Am·J·Physiol. 1998·Dec; 275(6‑2):R1725‑34. <·PMID:·9843861·> | Lin CW, Liu TY, Chen SU, Wang KT, Medeiros LJ, Hsu SM: CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features. Blood. 2005·Nov·15; 106(10):3567‑74. <·PMID:·16046525·> | Zhu DM, Dustin ML, Cairo CW, Thatte HS, Golan DE: Mechanisms of Cellular Avidity Regulation in CD2‑CD58‑Mediated T Cell Adhesion. ACS·Chem·Biol. 2006·Nov·21; 1(10):649‑58. <·PMID:·17168569·> | Pawlowski NN, Struck D, Grollich K, Kuhl AA, Zeitz M, Liesenfeld O, Hoffmann JC: CD2 deficiency partially prevents small bowel inflammation and improves parasite control in murine Toxoplasma gondii infection. World·J·Gastroenterol. 2007·Aug·21; 13(31):4207‑13. <·PMID:·17696249·>

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